Issue 8, 2017

Towards a correlative approach for characterising single virus particles by transmission electron microscopy and nanoscale Raman spectroscopy

Abstract

The morphology and structure of biological nanoparticles, such as viruses, can be efficiently analysed by transmission electron microscopy (TEM). To chemically characterise such nanoparticles in heterogeneous samples at the single particle level, we suggest tip-enhanced Raman spectroscopy (TERS) as a correlative method. Here we describe a TERS-compatible staining procedure for TEM which involves sample pre-scanning by TEM imaging, nanoparticle relocalisation by atomic force microscopy (AFM) followed by spectroscopic characterization of the virus nanoparticles using TERS. First successful correlative measurements are demonstrated on tobacco mosaic virus particles deposited on silicon-based TEM sample supports. In addition, the advantages and problems of this methodology are discussed.

Graphical abstract: Towards a correlative approach for characterising single virus particles by transmission electron microscopy and nanoscale Raman spectroscopy

Article information

Article type
Paper
Submitted
23 Leo 2016
Accepted
20 Ube 2017
First published
20 Ube 2017

Analyst, 2017,142, 1342-1349

Towards a correlative approach for characterising single virus particles by transmission electron microscopy and nanoscale Raman spectroscopy

A. Hermelink, D. Naumann, J. Piesker, P. Lasch, M. Laue and P. Hermann, Analyst, 2017, 142, 1342 DOI: 10.1039/C6AN02151D

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