Issue 6, 2011
From the journal:

Lab on a Chip

Controlling the contents of microdroplets by exploiting the permeability of PDMS†

Jung-uk Shim,‡ab   Santoshkumar N. Patil,‡a   James T. Hodgkinson,ab   Steven D. Bowden,b   David R. Spring,a   Martin Welch,b   Wilhelm T.S. Huck,*ac   Florian Hollfelder*b  and  Chris Abell*a  

* Corresponding authors

a Department of Chemistry, University of Cambridge, Cambridge, UK
E-mail: ca26@cam.ac.uk

b Department of Biochemistry, University of Cambridge, Cambridge, UK
E-mail: fh111@cam.ac.uk

c Radboud University Nijmegen, Institute for Molecules and Materials, Nijmegen, The Netherlands
E-mail: w.huck@science.ru.nl
Tel: +44 1223 336405

Abstract

A microfluidic device capable of exploiting the permeability of small molecules through polydimethylsiloxane (PDMS) has been fabricated in order to control the contents of microdroplets stored in storage wells. We demonstrate that protein precipitation and crystallization can be triggered by delivery of ethanol from a reservoir channel, thus controlling the protein solubility in microdroplets. Likewise quorum sensing in bacteria was triggered by delivery of the auto-inducer N-(3-oxododecanoyl)-L-homoserine lactone (OdDHL) through the PDMS membrane of the device.

Graphical abstract: Controlling the contents of microdroplets by exploiting the permeability of PDMS

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Article information

DOI
https://doi.org/10.1039/C0LC00615G
Article type
Paper
Submitted
19 nov 2010
Accepted
20 jan 2011
First published
07 feb 2011

Lab Chip, 2011,11, 1132-1137

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Controlling the contents of microdroplets by exploiting the permeability of PDMS

J. Shim, S. N. Patil, J. T. Hodgkinson, S. D. Bowden, D. R. Spring, M. Welch, W. T.S. Huck, F. Hollfelder and C. Abell, Lab Chip, 2011, 11, 1132 DOI: 10.1039/C0LC00615G

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