Issue 8, 2023

The compact integration of a cascaded HCR circuit for highly reliable cancer cell discrimination

Abstract

The accurate identification of multiple biomarkers involved in disease plays a vital role in effectively distinguishing cancer cells from normal cells, facilitating reliable cancer diagnosis. Motivated by this knowledge, we have engineered a compact and clamped cascaded DNA circuit for specifically discriminating cancer cells from normal cells via the amplified multi-microRNA imaging strategy. The proposed DNA circuit combines the traditional cascaded DNA circuit with multiply localized responsive character through the elaboration of two super-hairpin reactants, thus concurrently streamlining the circuit components and realizing localization-intensified cascaded signal amplification. In parallel, the multiple microRNA-stimulated sequential activations of the compact circuit, combined with a handy logic operation, significantly elevated the cell-discriminating reliability. Applications of the present DNA circuit in vitro and in cellular imaging experiments were executed with expected results, therefore illustrating that our DNA circuit is useful for precise cell discrimination and further clinical diagnosis.

Graphical abstract: The compact integration of a cascaded HCR circuit for highly reliable cancer cell discrimination

Supplementary files

Article information

Article type
Edge Article
Submitted
07 okt 2022
Accepted
16 jan 2023
First published
24 jan 2023
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2023,14, 2159-2167

The compact integration of a cascaded HCR circuit for highly reliable cancer cell discrimination

P. Dong, R. Li, S. He, Q. Zhang, J. Shang, Y. Jiang, X. Liu and F. Wang, Chem. Sci., 2023, 14, 2159 DOI: 10.1039/D2SC05568F

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