Synthetic hyperacetylation of nucleosomal histones

Hidetoshi Kajino; Tomomi Nagatani; Miku Oi; Tomoya Kujirai; Hitoshi Kurumizaka; Atsuya Nishiyama; Makoto Nakanishi; Kenzo Yamatsugu; Shigehiro A. Kawashima; Motomu Kanai

doi:10.1039/D0CB00029A

Synthetic hyperacetylation of nucleosomal histones†

Hidetoshi Kajino,^a Tomomi Nagatani,^b Miku Oi,^a Tomoya Kujirai,^cd Hitoshi Kurumizaka,^cd Atsuya Nishiyama,

^b Makoto Nakanishi,^b Kenzo Yamatsugu,

*^a Shigehiro A. Kawashima*^a and Motomu Kanai

*^a

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* Corresponding authors

^a Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
E-mail: yamatsugu@mol.f.u-tokyo.ac.jp, skawashima@mol.f.u-tokyo.ac.jp, kanai@mol.f.u-tokyo.ac.jp

^b Division of Cancer Cell Biology, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shiroganedai, Minato-ku, Tokyo 108-8639, Japan

^c Institute for Quantitative Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan

^d JST-ERATO, KURUMIZAKA Chromatin Atlas, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan

Abstract

We report combinations of a DMAP-based catalyst and phenyl acetate with optimal electron density as a new chemical system for high-yield, selective synthetic acetylation of histone lysine residues. The utility of this chemical system as a unique biologic tool is demonstrated by applying it to Xenopus laevis sperm chromatin.

This article is part of the themed collections: RSC Chemical Biology Transparent Peer Review Collection and International Open Access Week 2020

RSC Chemical Biology

Synthetic hyperacetylation of nucleosomal histones†

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