Issue 20, 2018

Quercetin spectrofluorometric quantification in aqueous media using different surfactants as fluorescence promoters

Abstract

Quercetin spectrofluorometric quantification was carried out in aqueous media (pH 7) using micelles of surfactants, namely: CTAB, SDS and TX100 as promoters of quercetin fluorescence, having a critical micelle concentration, CMC, of: 0.94 ± 0.03, 7.7 ± 0.6 and 0.18 ± 0.3 mM, respectively, measured through UV-Vis spectrophotometry. The thermodynamic binding constant, K, for the quercetin-surfactants' micelles supramolecular complex was estimated to be (log(K/M−1)) = 2.87 ± 0.02, 2.78 ± 0.04 and 2.80 ± 0.04, respectively, from fluorescence spectrophotometry. With the aid of these parameters it was possible to construct species distribution diagrams and choose the experimental conditions where quercetin can be quantified in aqueous media from fluorescence measurements. The best lowest limit of detection (0.244 ± 0.092) μM was achieved with CTAB micelles while the best sensitivity (2.919 ± 0.054) M−1 corresponded to SDS.

Graphical abstract: Quercetin spectrofluorometric quantification in aqueous media using different surfactants as fluorescence promoters

Supplementary files

Article information

Article type
Paper
Submitted
07 feb 2018
Accepted
14 mar 2018
First published
20 mar 2018
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2018,8, 10980-10986

Quercetin spectrofluorometric quantification in aqueous media using different surfactants as fluorescence promoters

J. C. Alva-Ensastegui, M. Palomar-Pardavé, M. Romero-Romo and M. T. Ramírez-Silva, RSC Adv., 2018, 8, 10980 DOI: 10.1039/C8RA01213J

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements