Issue 25, 2015

The best of both worlds: active enzymes by grafting-to followed by grafting-from a protein

Abstract

Hydrophilic polymers were attached to lysozyme by a combination of grafting-to and grafting-from approaches using RAFT polymerization. A hydrophilic oligomer was synthesized, and attached to the protein. The protein–oligomer hybrid contained the RAFT end group, enabling chain extension in solution. Lysozyme maintained activity throughout this process.

Graphical abstract: The best of both worlds: active enzymes by grafting-to followed by grafting-from a protein

Supplementary files

Article information

Article type
Communication
Submitted
20 nov 2014
Accepted
10 dec 2014
First published
10 dec 2014

Chem. Commun., 2015,51, 5343-5346

Author version available

The best of both worlds: active enzymes by grafting-to followed by grafting-from a protein

R. Falatach, C. McGlone, M. S. Al-Abdul-Wahid, S. Averick, R. C. Page, J. A. Berberich and D. Konkolewicz, Chem. Commun., 2015, 51, 5343 DOI: 10.1039/C4CC09287B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements