Issue 54, 2014

Direct electrochemistry of cytochrome c immobilized on a graphene oxide–carbon nanotube composite for picomolar detection of hydrogen peroxide

Abstract

We describe the fabrication of an amperometric biosensor based on cytochrome c (Cyt c) immobilized graphene oxide–multiwalled carbon nanotube (GO–MWCNT) composite on a nano Au modified glassy carbon electrode for trace level detection of H2O2. Morphology and surface characterization of the nanocomposite reveal the successful formation of a highly conducting MWCNT network on the GO surface. Electrochemical impedance studies indicate a lower charge transfer resistance compared to the bare electrode. Cyclic voltammetry studies clearly demonstrate an enhanced direct electrochemistry of Cyt c with a high electron transfer rate constant (ks) value of 3.4 s−1. An amperometric H2O2 biosensor has been fabricated with an excellent current sensitivity of 0.533 μA pM−1 cm−2 and a very low detection limit of 27.7 pM. The fabricated sensor shows exceptional selectivity to H2O2 in the presence of a high concentration of some likely interferents. Moreover, the sensor exhibits high stability with appreciable repeatability and reproducibility.

Graphical abstract: Direct electrochemistry of cytochrome c immobilized on a graphene oxide–carbon nanotube composite for picomolar detection of hydrogen peroxide

Article information

Article type
Paper
Submitted
29 mar 2014
Accepted
11 jun 2014
First published
11 jun 2014

RSC Adv., 2014,4, 28229-28237

Author version available

Direct electrochemistry of cytochrome c immobilized on a graphene oxide–carbon nanotube composite for picomolar detection of hydrogen peroxide

B. Dinesh, V. Mani, R. Saraswathi and S. Chen, RSC Adv., 2014, 4, 28229 DOI: 10.1039/C4RA02789B

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