Issue 57, 2014

Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins

Abstract

We demonstrate high-density labelling of cellular DNA and RNA using click chemistry and perform confocal and super-resolution microscopy. We visualize the crescent and ring-like structure of densely packed RNA in nucleoli. We further demonstrate click chemistry with unnatural amino acids for super-resolution imaging of outer-membrane proteins of E. coli.

Graphical abstract: Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins

Supplementary files

Article information

Article type
Communication
Submitted
05 feb 2014
Accepted
27 jun 2014
First published
27 jun 2014
This article is Open Access
Creative Commons BY license

RSC Adv., 2014,4, 30462-30466

Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins

A. Raulf, C. K. Spahn, P. J. M. Zessin, K. Finan, S. Bernhardt, A. Heckel and M. Heilemann, RSC Adv., 2014, 4, 30462 DOI: 10.1039/C4RA01027B

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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