Volume 233, 2022

An ultra-low noise amplifier array system for high throughput single entity analysis

Abstract

Electrochemical measurements at the single entity level provide ultra-sensitive tools for the precise diagnosis and understanding of basic biological and chemical processes. By decoding current signatures, single-entity electrochemistry provides abundant information on charges, sizes, shapes, catalytic performances and compositions. The accuracy of single-entity electrochemistry highly relies on advanced instrumentation to achieve the amperometric resolution at the sub-picoampere level and the temporal resolution at the sub-microsecond level. Currently, it is still a challenge for paralleling amplifiers to allow low-noise and high bandwidth single-entity electrochemical measurements. Herein, we developed a low-noise four-channel electrochemical instrumentation that integrates an Au electrode array with amplifiers in the circuit board. With this amplifier array, we achieved a high bandwidth (>100 kHz) electrochemical measurement. The further practical experiments proved the capability of this amplifier array system in acquiring transient signals from both single-molecule detection with an aerolysin nanopore and single Pt nanoparticle catalysis during the dynamic collision process. Paired with appropriate microfluidic array systems, our instrumentation will enable an extraordinarily high-throughput feature for single-entity sensing.

Graphical abstract: An ultra-low noise amplifier array system for high throughput single entity analysis

Associated articles

Supplementary files

Article information

Article type
Paper
Submitted
17 avg 2021
Accepted
13 sep 2021
First published
16 sep 2021

Faraday Discuss., 2022,233, 33-43

An ultra-low noise amplifier array system for high throughput single entity analysis

C. Zhong, H. Ma, J. Wang, L. Zhang, Y. Ying, R. Wang, Y. Wan and Y. Long, Faraday Discuss., 2022, 233, 33 DOI: 10.1039/D1FD00055A

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