Volume 227, 2021

Biodegradation of graphene materials catalyzed by human eosinophil peroxidase

Abstract

Understanding the biodegradability of graphene materials by the action of oxidative enzymes secreted by immune cells is essential for developing applicable biomedical products based on these materials. Herein, we demonstrate the biodegradation of graphene oxide (GO) by recombinant eosinophil peroxidase (EPO) enzyme extracted from human eosinophils in the presence of a low concentration of hydrogen peroxide and NaBr. We compared the degradation capability of the enzyme on three different GO samples containing different degrees of oxygen functional groups on their graphenic lattices. EPO succeeded in degrading the three tested GO samples within 90 h treatment. Raman spectroscopy and transmission electron microscopy analyses provided clear-cut evidence for the biodegradation of GO by EPO catalysis. Our results provide more insight into a better understanding of the biodegradation of graphene materials, helping the design of future biomedical products based on these carbon nanomaterials.

Graphical abstract: Biodegradation of graphene materials catalyzed by human eosinophil peroxidase

Associated articles

Article information

Article type
Paper
Submitted
29 sep 2019
Accepted
24 jan 2020
First published
31 jan 2020

Faraday Discuss., 2021,227, 189-203

Biodegradation of graphene materials catalyzed by human eosinophil peroxidase

R. Kurapati, C. Martìn, V. Palermo, Y. Nishina and A. Bianco, Faraday Discuss., 2021, 227, 189 DOI: 10.1039/C9FD00094A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements