Droplet microfluidic system for high throughput and passive selection of bacteria producing biosurfactants

Klaudia Staskiewicz, Maria Dabrowska-Zawada, Lukasz Kozon, Zofia Olszewska, Lukasz Drewniak and Tomasz S. Kaminski

Lab Chip, 2024, 24, 1947
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Peptide–based artificial cytoskeleton enhances colocalized cascade reactions in cell-like microreactors

Thao P. Doan-Nguyen, Shoupeng Cao, Tsvetomir Ivanov, Katharina Landfester and Lucas Caire da Silva

Chem. Sci., 2026, 17, 7707
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Time-resolved single-cell secretion analysis via microfluidics

Ying Xu, Mei Tsz Jewel Chan, Ming Yang, Heixu Meng and Chia-Hung Chen

Lab Chip, 2025, 25, 1282
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Studying the evolutionary potential of ancestral aryl sulfatases in the alkaline phosphatase family with droplet microfluidics

Bernard D. G. Eenink, Josephin M. Holstein, Magdalena Heberlein, Carina Dilkaute, Joachim Jose, Florian Hollfelder, Bert van Loo, Erich Bornberg-Bauer, Tomasz S. Kaminski and Andreas Lange

Analyst, 2026, 151, 1794
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Recent trends in biocatalysis

Dong Yi, Thomas Bayer, Christoffel P. S. Badenhorst, Shuke Wu, Mark Doerr, Matthias Höhne and Uwe T. Bornscheuer

Chem. Soc. Rev., 2021, 50, 8003
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Ultra-high throughput droplet microfluidics for cultivation and functional screening of environmental microbial strains and consortia

Luca Potenza, Jozef Krzak, Maciej S. Andrzejewski, Adam Pyzik and Tomasz S. Kaminski

Lab Chip, 2026, 26, 2646
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Ultrahigh‐Throughput Detection of Enzymatic Alcohol Dehydrogenase Activity in Microfluidic Droplets with a Direct Fluorogenic Assay

Miriam Klaus, Paul Jannis Zurek, Tomasz S. Kaminski, Ahir Pushpanath, Katharina Neufeld and Florian Hollfelder

ChemBioChem, 2021, 22, 3292
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Recent developments of droplets-based microfluidics for bacterial analysis

Ruizhi Ning, Jinhai Fan, Liang Kong, Xue Jiang, Yun Qian, Tao Du, Guangjian Zhang and Weiwei Wu

Chinese Chemical Letters, 2022, 33, 2243
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Droplet microfluidic multistep platform for high-throughput active selection of cellulolytic microorganisms

Luca Potenza, Lukasz Kozon, Lukasz Drewniak and Tomasz S. Kaminski

Sensors and Actuators Reports, 2026, 11, 100468
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A Microfluidics-Based Ultrahigh-Throughput Screening Unveils Diverse Ketoreductases Relevant to Pharmaceutical Synthesis

Laura Blas-Muñoz, Alejandro H. Orrego, Michael Hofmeister, John Martínez-Salvador, Carmen Ortega, Vanessa Rondón Berrio, Jorge Díaz-Rullo, James Finnigan, Simon Charnock, Wolf-Dieter Fessner, José Eduardo González-Pastor and Aurelio Hidalgo

Anal. Chem., 2025, 97, 20698
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The use of droplet-based microfluidic technologies for accelerated selection of Yarrowia lipolytica and Phaffia rhodozyma yeast mutants

Taras Mika, Martins Kalnins and Kriss Spalvins

Biology Methods and Protocols, 2024, 9
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Ultra-High-Throughput Absorbance-Activated Droplet Sorting for Enzyme Screening at Kilohertz Frequencies

Elliot J. Medcalf, Maximilian Gantz, Tomasz S. Kaminski and Florian Hollfelder

Anal. Chem., 2023, 95, 4597
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Recent advances in droplet sequential monitoring methods for droplet sorting

Yukun He, Yi Qiao, Lu Ding, Tianguang Cheng and Jing Tu

Biomicrofluidics, 2023, 17
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Confocal Absorbance‐Activated Droplet Sorting (cAADS) for Enzyme Engineering

Abdi Mirgissa Kaba, Sébastien Gounel, Thomas Beneyton, Lionel Buisson, Jean‐Christophe Baret and Nicolas Mano

Advanced Science, 2025, 12
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Label-free droplet-based bacterial growth phenotype screening by a mini integrated microfluidic platform

Anle Ge, Zhidian Diao, Fengyi Liu, Yuandong Li, Chaojie Cui, Shoujie Li, Yang Liu, Jian Xu and Bo Ma

Sensors and Actuators B: Chemical, 2023, 385, 133691
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Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

Maximilian Gantz, Stefanie Neun, Elliot J. Medcalf, Liisa D. van Vliet and Florian Hollfelder

Chem. Rev., 2023, 123, 5571
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Recent Advances on Sorting Methods of High-Throughput Droplet-Based Microfluidics in Enzyme Directed Evolution

Xiaozhi Fu, Yueying Zhang, Qiang Xu, Xiaomeng Sun and Fanda Meng

Front. Chem., 2021, 9
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Passive Droplet Microfluidic Platform for High-Throughput Screening of Microbial Proteolytic Activity

Luca Potenza, Lukasz Kozon, Lukasz Drewniak and Tomasz S. Kaminski

Anal. Chem., 2024, 96, 15931
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Droplet-Based Microfluidics in Single-Bacterium Analysis: Advancements in Cultivation, Detection, and Application

Haiyan Ma, Yuewen Zhang, Ren Shen and Yanwei Jia

Biosensors, 2025, 15, 535
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TriD-LAMP: A pump-free microfluidic chip for duplex droplet digital loop-mediated isothermal amplification analysis

Cui Wu, Linbo Liu, Zunzhong Ye, Jingjing Gong, Pei Hao, Jianfeng Ping and Yibin Ying

Analytica Chimica Acta, 2022, 1233, 340513
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Versatile Product Detection via Coupled Assays for Ultrahigh-Throughput Screening of Carbohydrate-Active Enzymes in Microfluidic Droplets

Simon Ladeveze, Paul J. Zurek, Tomasz S. Kaminski, Stephane Emond and Florian Hollfelder

ACS Catal., 2023, 13, 10232
DOI: 10.1021/acscatal.3c01609

Induced droplet ovalization (IDO): Image-based microfluidic method for high-throughput and label-free characterization of microbial proteolytic strains from wastewater sludge

Luca Potenza, Maciej S. Andrzejewski and Tomasz S. Kaminski

Sensors and Actuators B: Chemical, 2025, 445, 138554
DOI: 10.1016/j.snb.2025.138554

Droplet digital polymerase chain reaction: Microfluidic advances and biomedical applications

Kailiang Zhang and Zhijie Xie

Physics of Fluids, 2026, 38
DOI: 10.1063/5.0315030

A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalized Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution

Che Fai Alex Wong, Liisa van Vliet, Swapnil Vilas Bhujbal, Chengzhi Guo, Marit Sletmoen, Bjørn Torger Stokke, Florian Hollfelder and Rahmi Lale

ACS Synth. Biol., 2021, 10, 1882
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Research advances in the application of microfluidic chip technology for rapid detection of antibiotic-resistant bacteria

Lijun Xia, Changqing Tang, Dalong Tong and Qifu He

Front. Cell. Infect. Microbiol., 2026, 16
DOI: 10.3389/fcimb.2026.1819856

Helping proteins come in from the cold: 5 burning questions about cold-active enzymes

Jan Stanislaw Nowak and Daniel E. Otzen

BBA Advances, 2024, 5, 100104
DOI: 10.1016/j.bbadva.2023.100104

Vector redesign and in‐droplet cell‐growth improves enrichment and recovery in live Escherichia coli

Bernard D. G. Eenink, Tomasz S. Kaminski, Erich Bornberg‐Bauer, Joachim Jose, Florian Hollfelder and Bert van Loo

Microbial Biotechnology, 2022, 15, 2845
DOI: 10.1111/1751-7915.14144