Issue 23, 2024

Oleic acid differentially affects lipid droplet storage of de novo synthesized lipids in hepatocytes and adipocytes

Abstract

Lipogenesis is a vital but often dysregulated metabolic pathway. Here we use optical photothermal infrared imaging to quantify lipogenesis rates of isotopically labelled oleic acid and glucose concomitantly in live cells. In hepatocytes, but not adipocytes, we find that oleic acid feeding at 60 μM increases the number and size of lipid droplets (LDs) while simultaneously inhibiting storage of de novo synthesized lipids in LDs. Our results demonstrate alternate regulation of lipogenesis between cell types.

Graphical abstract: Oleic acid differentially affects lipid droplet storage of de novo synthesized lipids in hepatocytes and adipocytes

Supplementary files

Article information

Article type
Communication
Submitted
04 okt 2023
Accepted
29 jan 2024
First published
30 jan 2024
This article is Open Access
Creative Commons BY license

Chem. Commun., 2024,60, 3138-3141

Oleic acid differentially affects lipid droplet storage of de novo synthesized lipids in hepatocytes and adipocytes

H. B. Castillo, S. O. Shuster, L. H. Tarekegn and C. M. Davis, Chem. Commun., 2024, 60, 3138 DOI: 10.1039/D3CC04829B

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