Issue 56, 2021

CRISPR/Cas12a-mediated gold nanoparticle aggregation for colorimetric detection of SARS-CoV-2

Abstract

The trans-cleavage activity of the target-activated CRISPR/Cas12a liberated an RNA crosslinker from a molecular transducer, which facilitated the assembly of gold nanoparticles. Integration of the molecular transducer with isothermal amplification and CRISPR/Cas12a resulted in visual detection of the N gene and E gene of SARS-CoV-2 in 45 min.

Graphical abstract: CRISPR/Cas12a-mediated gold nanoparticle aggregation for colorimetric detection of SARS-CoV-2

Supplementary files

Article information

Article type
Communication
Submitted
14 maj 2021
Accepted
17 jun 2021
First published
18 jun 2021
This article is Open Access
Creative Commons BY license

Chem. Commun., 2021,57, 6871-6874

CRISPR/Cas12a-mediated gold nanoparticle aggregation for colorimetric detection of SARS-CoV-2

Y. Cao, J. Wu, B. Pang, H. Zhang and X. C. Le, Chem. Commun., 2021, 57, 6871 DOI: 10.1039/D1CC02546E

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