Issue 5, 2019

A highly sensitive electrochemical aptasensor for detection of microcystin-LR based on a dual signal amplification strategy

Abstract

In this work, a sensitive and selective electrochemical aptasensor for determination of microcystin-LR (MC-LR) was developed based on a dual signal amplification system consisting of a novel ternary composite and horseradish peroxidase (HRP). The ternary composite was prepared by depositing gold nanoparticles (AuNPs) on molybdenum disulfide (MoS2) covered TiO2 nanobeads (TiONBs). MoS2 nanosheet modified TiONBs provided a large surface area for immobilization of AuNPs and biomolecules. The ternary composite also possesses an improved electron transfer and catalytic capability. To construct the aptasensor, thiolated MC-LR aptamers were immobilized on the AuNP@MoS2-TiONB modified electrode through a gold–sulfur bond. Then, biotin–cDNA with a sequence complementary to the MC-LR aptamer competed with MC-LR for binding to the immobilized aptamer. The current signal catalyzed by avidin–HRP decreased with the increase of MC-LR, based on which a linear range of 0.005–30 nM and a detection limit of 0.002 nM were obtained.

Graphical abstract: A highly sensitive electrochemical aptasensor for detection of microcystin-LR based on a dual signal amplification strategy

Article information

Article type
Paper
Submitted
14 okt 2018
Accepted
31 dec 2018
First published
03 jan 2019

Analyst, 2019,144, 1671-1678

A highly sensitive electrochemical aptasensor for detection of microcystin-LR based on a dual signal amplification strategy

X. Liu, Y. Tang, P. Liu, L. Yang, L. Li, Q. Zhang, Y. Zhou and Md. Z. H. Khan, Analyst, 2019, 144, 1671 DOI: 10.1039/C8AN01971A

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