Issue 24, 2017

Bulk double emulsification for flow cytometric analysis of microfluidic droplets

Abstract

Droplet microfluidics is valuable for applications in chemistry and biology, but generates massive numbers of droplets that must be analyzed and sorted. Here, we describe a simple approach to bulk double emulsify microfluidic emulsions for analysis and sorting with commercial flow cytometers. We illustrate the method by using it to identify droplets based on nucleic acid content. Though simple, our method provides a general approach for analyzing and sorting microfluidic droplets without custom microfluidic double emulsifiers or sorters.

Graphical abstract: Bulk double emulsification for flow cytometric analysis of microfluidic droplets

Article information

Article type
Communication
Submitted
13 okt 2017
Accepted
16 okt 2017
First published
13 nov 2017
This article is Open Access
Creative Commons BY-NC license

Analyst, 2017,142, 4618-4622

Bulk double emulsification for flow cytometric analysis of microfluidic droplets

D. J. Sukovich, S. C. Kim, N. Ahmed and A. R. Abate, Analyst, 2017, 142, 4618 DOI: 10.1039/C7AN01695F

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