Issue 7, 2015

Rapid biodiagnostic ex vivo imaging at 1 μm pixel resolution with thermal source FTIR FPA

Abstract

A recent upgrade to the optics configuration of a thermal source FTIR microscope equipped with a focal plane array detector has enabled rapid acquisition of high magnification spectrochemical images, in transmission, with an effective geometric pixel size of ∼1 × 1 μm2 at the sample plane. Examples, including standard imaging targets for scale and accuracy, as well as biomedical tissues and microorganisms, have been imaged with the new system and contrasted with data acquired at normal magnification and with a high magnification multi-beam synchrotron instrument. With this optics upgrade, one can now conduct rapid biodiagnostic ex vivo tissue imaging in-house, with images collected over larger areas, in less time (minutes) and with comparable quality and resolution to the best synchrotron source FTIR imaging capabilities.

Graphical abstract: Rapid biodiagnostic ex vivo imaging at 1 μm pixel resolution with thermal source FTIR FPA

Article information

Article type
Paper
Submitted
30 okt 2014
Accepted
07 jan 2015
First published
07 jan 2015
This article is Open Access
Creative Commons BY license

Analyst, 2015,140, 2493-2503

Rapid biodiagnostic ex vivo imaging at 1 μm pixel resolution with thermal source FTIR FPA

C. R. Findlay, R. Wiens, M. Rak, J. Sedlmair, C. J. Hirschmugl, J. Morrison, C. J. Mundy, M. Kansiz and K. M. Gough, Analyst, 2015, 140, 2493 DOI: 10.1039/C4AN01982B

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