Single cell sequencing: An engineer and business person's perspective on how we got here
Microfluidics entrepreneur Mark Gilligan provides a perspective on the development of single-cell sequencing technologies.
InDrops and Drop-seq technologies for single-cell sequencing
Droplet-based single-cell sequencing pioneers Allon Klein and Evan Macosko summarise the growth of the technology and look ahead to future developments.
Perspective on droplet-based single-cell sequencing
Thought leader David Weitz introduces the Lab on a Chip droplet-based single-cell sequencing thematic collection.
Droplet-based single cell RNAseq tools: a practical guide
A step-by-step guide for droplet-based single cell RNAseq experiments, practical considerations and technical notes.
Finding a helix in a haystack: nucleic acid cytometry with droplet microfluidics
Nucleic acid cytometry using droplet microfluidics identifies and sorts single cells, virus, or free molecules based on specific “keyword” sequences.
High throughput gene expression profiling of yeast colonies with microgel-culture Drop-seq
We describe isogenic colony sequencing (ICO-seq), a massively-parallel strategy to assess the gene expression profiles of large numbers of genetically distinct yeast colonies.
Paper
Simplified Drop-seq workflow with minimized bead loss using a bead capture and processing microfluidic chip
Single-cell RNA-sequencing (scRNA-seq) has revolutionized biomedical research by enabling the in-depth analysis of cell-to-cell heterogeneity of tissues with unprecedented resolution.
Functional TCR T cell screening using single-cell droplet microfluidics
Droplet-based single cell platform allows functional screening and sorting of desirable TCR T cells to accelerate development of adoptive T cell therapies.
Ultrahigh-throughput droplet microfluidic device for single-cell miRNA detection with isothermal amplification
An ultrahigh-throughput single-cell miRNA assay is developed by a continuous-flow microfluidic process employing isothermal amplification to amplify the target miRNA signal.
Inertial-ordering-assisted droplet microfluidics for high-throughput single-cell RNA-sequencing
We developed a modified high-throughput droplet barcoding technique for single-cell Drop-Seq via introduction of hydrodynamic ordering in a spiral microchannel.
About this collection
The field of droplet-based single-cell sequencing field has made increasing advances in recent years. Large numbers of studies are underway to collect and explore the new information that is now accessible with single-cell RNA-seq. Improvements to microfluidics are advancing rapidly and extensions to other sequencing methods are also being developed, enabling investigations to probe information beyond mRNA alone. This has rapidly become a burgeoning field, where microfluidic techniques are essential and where droplet-based microfluidics has enabled a major advance. The collection highlights research focussing on the interface between the technological advancements and high impact applications of droplet-based single-cell sequencing. This on-going collection is collated by Thought Leader David Weitz and the Lab on a Chip Editorial Office.
More details about the collection and how to apply can be found at rsc.li/drop-sc-seq-blog