Re-investigation of the HExxH enzyme DarF reveals a dehydrogenation–epoxidation reaction

Jabal Rahmat Haedar, Nils Böhringer, Gaja Swarna Kumari, Vic Kiselov, Viktors Romanuks, Gints Smits, Stefano Donadio, Till F. Schäberle and Chin-Soon Phan

Org. Biomol. Chem., 2026
DOI: 10.1039/D6OB00496B

Phylogeny-guided discovery of a promiscuous P450 macrocyclase for the production of diverse atropopeptides

Bin Tan, Peter Breunig, Lamia Arbib, Yuya Kakumu, Friederike Biermann, Kornelia Hardes, Jasmin Hefendehl and Eric J. N. Helfrich

Chem. Sci., 2025, 16, 16240
DOI: 10.1039/D5SC03525B

Recent advances in peptide macrocyclization strategies

Pengyuan Fang, Wing-Ka Pang, Shouhu Xuan, Wai-Lun Chan and Ken Cham-Fai Leung

Chem. Soc. Rev., 2024, 53, 11725
DOI: 10.1039/D3CS01066J

syn-Elimination of glutamylated threonine in lanthipeptide biosynthesis

Raymond Sarksian, Lingyang Zhu and Wilfred A. van der Donk

Chem. Commun., 2023, 59, 1165
DOI: 10.1039/D2CC06345J

Remodeling of ribosomally synthesized peptide backbones based on posttranslational modifications

Yanqing Xue, Yijiao Xiong, Wei Huang, Jianing Liu and Wen Liu

Nat. Prod. Rep., 2025, 42, 1276
DOI: 10.1039/D5NP00018A

Selective editing of a peptide skeleton via C–N bond formation at N-terminal aliphatic side chains

Yujie Han, Junjie Shi, Songrong Li, Tingting Dan, Wenwen Yang and Mingyu Yang

Chem. Sci., 2022, 13, 14382
DOI: 10.1039/D2SC04909K

Hyalucidin A: A Bacterial RiPP Featuring a Benzofuranoindoline Motif Biosynthesized by Tandem P450 Catalysis

Fang Zhou Yin, Zi Jie Wang, Yi Ling Hu, Cheng Li Liu, Zhi Kai Guo, Ren Xiang Tan, Jing Shi and Hui Ming Ge

Angew Chem Int Ed, 2026, 65
DOI: 10.1002/anie.202522698

Hyalucidin A: A Bacterial RiPP Featuring a Benzofuranoindoline Motif Biosynthesized by Tandem P450 Catalysis

Fang Zhou Yin, Zi Jie Wang, Yi Ling Hu, Cheng Li Liu, Zhi Kai Guo, Ren Xiang Tan, Jing Shi and Hui Ming Ge

Angewandte Chemie, 2026, 138
DOI: 10.1002/ange.202522698

Radical SAM Enzyme WprB Catalyzes Uniform Cross-Link Topology between Trp-C5 and Arg-Cγ on the Precursor Peptide

Abujunaid Habib Khan, Jabal Rahmat Haedar, Vic Kiselov, Viktors Romanuks, Gints Smits, Stefano Donadio and Chin-Soon Phan

ACS Chem. Biol., 2025, 20, 259
DOI: 10.1021/acschembio.4c00693

P450-Modified Ribosomally Synthesized Peptides with Aromatic Cross-Links

Yi Ling Hu, Fang Zhou Yin, Jing Shi, Shi Ying Ma, Zi Ru Wang, Ren Xiang Tan, Rui Hua Jiao and Hui Ming Ge

J. Am. Chem. Soc., 2023, 145, 27325
DOI: 10.1021/jacs.3c07416

Complex peptide natural products: Biosynthetic principles, challenges and opportunities for pathway engineering

Sebastian L. Wenski, Sirinthra Thiengmag and Eric J.N. Helfrich

Synthetic and Systems Biotechnology, 2022, 7, 631
DOI: 10.1016/j.synbio.2022.01.007

Chemical synthesis and biosynthesis of darobactin

Xinhui Li, Suze Ma and Qi Zhang

Tetrahedron Letters, 2023, 116, 154337
DOI: 10.1016/j.tetlet.2023.154337

Engineering lanthipeptides by introducing a large variety of RiPP modifications to obtain new-to-nature bioactive peptides

Yuxin Fu, Yanli Xu, Fleur Ruijne and Oscar P Kuipers

FEMS Microbiology Reviews, 2023, 47
DOI: 10.1093/femsre/fuad017

Promiscuity of Omphalotin A Biosynthetic Enzymes Allows de novo Production of Non‐Natural Multiply Backbone N‐Methylated Peptide Macrocycles in Yeast

Emmanuel Matabaro, Luca Witte, Fabio Gherlone, Eva Vogt, Hannelore Kaspar and Markus Künzler

ChemBioChem, 2024, 25
DOI: 10.1002/cbic.202300626

Fungal RiPPs Side Chain Macrocyclization Catalyzed by Copper-Dependent DUF3328 Enzyme

Chen-Yu Chiang, Masao Ohashi and Yi Tang

J. Am. Chem. Soc., 2025, 147, 8113
DOI: 10.1021/jacs.4c18770

Structural and mechanistic insights into iminium-catalysed macrocyclization by nuclear transport factor 2-like enzymes

Cheng Li Liu, Bo Zhang, Cheng Yuan Yuan, Zi Jie Wang, Ao Zhu, Si Yuan Wang, Zhang Yuan Yan, Andrey Lebedev, Yasuhiro Igarashi, Ren Xiang Tan and Hui Ming Ge

Nat. Synth, 2026, 5, 775
DOI: 10.1038/s44160-025-00989-z

Mechanistic Insights into the Selective C–S Bond Formation by P450 TleB

Hongxun Gao, Yakun Fan, Xuwei He, Xiaogang Peng, Zhen Li, Yanxin Zheng, Shengbiao Ji, Longwu Ye, Aitao Li, Binju Wang and Jing Zhao

ACS Catal., 2024, 14, 10658
DOI: 10.1021/acscatal.4c03328

Daropeptide natural products

Suze Ma, Sijia Guo, Wei Ding and Qi Zhang

Explor Drug Sci, 2024, 190
DOI: 10.37349/eds.2024.00042

Biocatalytic synthesis of peptidic natural products and related analogues

Dake Liu, Garret M. Rubin, Dipesh Dhakal, Manyun Chen and Yousong Ding

iScience, 2021, 24, 102512
DOI: 10.1016/j.isci.2021.102512

P450 Biarylitide Synthase ShyB Catalyzes the Cross-Link Between His-C2 and Tyr-O4 on the Precursor Peptide

Abujunaid Habib Khan, Jabal Rahmat Haedar, Vic Kiselov, Viktors Romanuks, Gints Smits, Stefano Donadio and Chin-Soon Phan

Org. Lett., 2025, 27, 5715
DOI: 10.1021/acs.orglett.5c01487

Bacterial Cytochrome P450 Catalyzed Macrocyclization of Ribosomal Peptides

Jing Liu, Runze Liu, Bei-Bei He, Xiaoqian Lin, Longcheng Guo, Gengfan Wu and Yong-Xin Li

ACS Bio Med Chem Au, 2024, 4, 268
DOI: 10.1021/acsbiomedchemau.4c00080

Aminovinyl Cysteine Containing Peptides: A Unique Motif That Imparts Key Biological Activity

Emily S. Grant-Mackie, Elyse T. Williams, Paul W. R. Harris and Margaret A. Brimble

JACS Au, 2021, 1, 1527
DOI: 10.1021/jacsau.1c00308

P450 Cyptide Synthase KwwB Catalyzes Trp-C5–Trp-N1 Cross-Linking and Accepts Diverse Precursor Peptides

Jabal Rahmat Haedar, Gaja Swarna Kumari, Vic Kiselov, Viktors Romanuks, Gints Smits, Stefano Donadio and Chin-Soon Phan

Org. Lett., 2026, 28, 433
DOI: 10.1021/acs.orglett.5c04848

Tryptophan-specific peptide modification through metal-free photoinduced N-H alkylation employing N-aryl glycines

Jianhui Yin, Wenjing Huang, Changyong Guo, Chao Liu, Fei Gao and Honggang Hu

Chinese Chemical Letters, 2024, 35, 109244
DOI: 10.1016/j.cclet.2023.109244

Substrate Recognition by the Peptidyl-(S)-2-mercaptoglycine Synthase TglHI during 3-Thiaglutamate Biosynthesis

Martin I. McLaughlin, Yue Yu and Wilfred A. van der Donk

ACS Chem. Biol., 2022, 17, 930
DOI: 10.1021/acschembio.2c00087

A Unique Class of Cyclases with a Kinase Fold Catalyzes Enethiol-Mediated Macrocyclization of Aminovinyl-Cysteine Motifs in Lanthipeptides

Xiang-Qian Xie, Wen Guo, Yin-Zheng Xia, Li-Juan Liao, Meng-Xin Sun, Jing-Xue Wang, Jiang-Tao Gao, Hong-Wei Yao and Huan Wang

ACS Cent. Sci., 2025, 11, 1178
DOI: 10.1021/acscentsci.5c00569

Second-generation DNA-encoded multiple display on a constant macrocyclic scaffold enabled by an orthogonal protecting group strategy

Qigui Nie, Shuting Zhong, Yangfeng Li, Gong Zhang and Yizhou Li

Chinese Chemical Letters, 2022, 33, 2559
DOI: 10.1016/j.cclet.2021.09.041