The design strategies and biological applications of probes for the gaseous signaling molecule hydrogen sulfide

Abstract

H₂S, the smallest and simplest biological thiol in living systems, is the third member of the family of signaling mediators. H₂S participates in the regulation of a series of complex physiological and pathological functions in the body, making it a critical fulcrum that balances health and disease in human physiology. Small-molecule fluorescent probes have been proven to possess the unique advantages of high temporal and spatial resolution, good biocompatibility and high sensitivity, and thus their use is a powerful approach for monitoring the level and dynamics of H₂S in living cells and organisms and better understanding its basic cellular functions. The field of small-molecule fluorescent probes for monitoring the complex biological activities of H₂S in vivo has been thriving in recent years. Herein, we systematically summarize the latest developments in the field of fluorescent probes for the detection of H₂S, illustrate their biological applications according to the classification of target-responsive sites, and emphasize the development direction and challenges of H₂S-responsive fluorescent probes, hoping to give implications of researchers on fluorescent probes for future research.