Issue 13, 2015

Modulation of human IAPP fibrillation: cosolutes, crowders and chaperones

Abstract

The cellular environment determines the structure and function of proteins. Marginal changes of the environment can severely affect the energy landscape of protein folding. However, despite the important role of chaperones on protein folding, less is known about chaperonal modulation of protein aggregation and fibrillation considering different classes of chaperones. We find that the pharmacological chaperone O4, the chemical chaperone proline as well as the protein chaperone serum amyloid P component (SAP) are inhibitors of the type 2 diabetes mellitus-related aggregation process of islet amyloid polypeptide (IAPP). By applying biophysical methods such as thioflavin T fluorescence spectroscopy, fluorescence anisotropy, total reflection Fourier-transform infrared spectroscopy, circular dichroism spectroscopy and atomic force microscopy we analyse and compare their inhibition mechanism. We demonstrate that the fibrillation reaction of human IAPP is strongly inhibited by formation of globular, amorphous assemblies by both, the pharmacological and the protein chaperones. We studied the inhibition mechanism under cell-like conditions by using the artificial crowding agents Ficoll 70 and sucrose. Under such conditions the suppressive effect of proline was decreased, whereas the pharmacological chaperone remains active.

Graphical abstract: Modulation of human IAPP fibrillation: cosolutes, crowders and chaperones

Article information

Article type
Paper
Submitted
15 okt 2014
Accepted
11 nov 2014
First published
11 nov 2014

Phys. Chem. Chem. Phys., 2015,17, 8338-8348

Author version available

Modulation of human IAPP fibrillation: cosolutes, crowders and chaperones

M. Gao, K. Estel, J. Seeliger, R. P. Friedrich, S. Dogan, E. E. Wanker, R. Winter and S. Ebbinghaus, Phys. Chem. Chem. Phys., 2015, 17, 8338 DOI: 10.1039/C4CP04682J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements