Issue 12, 2015
From the journal:

Chemical Communications

Biocatalytic conversion of ethylene to ethylene oxide using an engineered toluene monooxygenase†

D. A. Carlin,a   S. J. Bertolanib  and  J. B. Siegel*bcd  

* Corresponding authors

a Biophysics Graduate Group, University of California Davis, Davis, California, USA

b Department of Chemistry, University of California Davis, Davis, California, USA
E-mail: jbsiegel@ucdavis.edu

c Department of Biochemistry and Molecular Medicine, University of California Davis, Davis, California, USA

d Genome Center, University of California Davis, Davis, California, USA

Abstract

Mutants of toluene o-xylene monooxygenase are demonstrated to oxidize ethylene to ethylene oxide in vivo at yields of >99%. The best mutant increases ethylene oxidation activity by >5500-fold relative to the native enzyme. This is the first report of a recombinant enzyme capable of carrying out this industrially significant chemical conversion.

Graphical abstract: Biocatalytic conversion of ethylene to ethylene oxide using an engineered toluene monooxygenase

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Article information

DOI
https://doi.org/10.1039/C4CC08802F
Article type
Communication
Submitted
04 nov 2014
Accepted
12 dec 2014
First published
05 jan 2015

Chem. Commun., 2015,51, 2283-2285

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Biocatalytic conversion of ethylene to ethylene oxide using an engineered toluene monooxygenase

D. A. Carlin, S. J. Bertolani and J. B. Siegel, Chem. Commun., 2015, 51, 2283 DOI: 10.1039/C4CC08802F

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