Issue 24, 2024

Prudently designed Se@fMWCNT as a peroxidase mimicking nanozyme for distinctive electrochemical detection of H2O2 and glutathione

Abstract

Nanozymes are nanomaterials with enzymatic characteristics that are used to overcome the challenges associated with naturally occurring enzymes. A peroxidase mimicking nanozyme has been developed in this work, which has ability in the reduction of hydrogen peroxide (H2O2) to water, by oxidising the substrates in the presence of H2O2. We have designed and developed a neoteric Se@fMWCNT nanocomposite with peroxidase mimicking activity for electrochemical detection of H2O2 and glutathione (GSH). The choice of selenium (Se) was inspired by the natural enzymatic antioxidant glutathione peroxidase, which has Se as its active centre. Before preparation of the nanocomposite, MWCNT was functionalized with acid to obtain functionalized MWCNT, in order to improve biocompatibility and to increase conductivity by providing abundant active sites for the successful incorporation of Se. The as-synthesized nanocomposite was immobilized on a glassy carbon electrode, which could then be used for amperometric detection of the analytes H2O2 and GSH at neutral pH. The fabricated sensor exhibits a linear detection range of 50 nM–1.4 μM and a limit of detection (LOD) of 18.23 nM for H2O2, and a range of 50–450 μM and 500 μM–1.5 mM for GSH determination, with an LOD of 19.2 μM.

Graphical abstract: Prudently designed Se@fMWCNT as a peroxidase mimicking nanozyme for distinctive electrochemical detection of H2O2 and glutathione

Supplementary files

Article information

Article type
Paper
Submitted
27 mar 2024
Accepted
20 máj 2024
First published
22 máj 2024

J. Mater. Chem. C, 2024,12, 8924-8934

Prudently designed Se@fMWCNT as a peroxidase mimicking nanozyme for distinctive electrochemical detection of H2O2 and glutathione

V. P. Sruthi and S. Senthilkumar, J. Mater. Chem. C, 2024, 12, 8924 DOI: 10.1039/D4TC01231C

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