Environmentally relevant concentrations of titanium dioxide nanoparticles pose negligible risk to marine microbes

Abstract

Nano-sized titanium dioxide (nTiO₂) represents the highest produced nanomaterial by mass worldwide and, due to its prevalent industrial and commercial use, it inevitably reaches the natural environment. Previous work has revealed a negative impact of nTiO₂ upon marine phytoplankton growth, however, studies are typically carried out at concentrations far exceeding those measured and predicted to occur in the environment currently. Here, a series of experiments were carried out to assess the effects of both research-grade nTiO₂ and nTiO₂ extracted from consumer products upon the marine dominant cyanobacterium, Prochlorococcus, and natural marine communities at environmentally relevant and supra-environmental concentrations (i.e., 1 μg L⁻¹ to 100 mg L⁻¹). Cell declines observed in Prochlorococcus cultures were associated with the extensive aggregation behaviour of nTiO₂ in saline media and the subsequent entrapment of microbial cells. Hence, higher concentrations of nTiO₂ particles exerted a stronger decline of cyanobacterial populations. However, within natural oligotrophic seawater, cultures were able to recover over time as the nanoparticles aggregated out of solution after 72 h. Subsequent shotgun proteomic analysis of Prochlorococcus cultures exposed to environmentally relevant concentrations confirmed minimal molecular features of toxicity, suggesting that direct physical effects are responsible for short-term microbial population decline. In an additional experiment, the diversity and structure of natural marine microbial communities showed negligible variations when exposed to environmentally relevant nTiO₂ concentrations (i.e., 25 μg L⁻¹). As such, the environmental risk of nTiO₂ towards marine microbial species appears low, however the potential for adverse effects in hotspots of contamination exists. In future, research must be extended to consider any effect of other components of nano-enabled product formulations upon nanomaterial fate and impact within the natural environment.