Ratiometric DNA sensing with a host–guest FRET pair

Shuai Zhang; Khaleel I. Assaf; Chusen Huang; Andreas Hennig; Werner M. Nau

doi:10.1039/C8CC09126A

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Ratiometric DNA sensing with a host–guest FRET pair†

Shuai Zhang,

^a Khaleel I. Assaf,

^a Chusen Huang,^a Andreas Hennig

*^a and Werner M. Nau

*^a

Author affiliations

* Corresponding authors

^a Department of Life Sciences and Chemistry, Jacobs University Bremen, Campus Ring 1, Bremen, Germany
E-mail: a.hennig@jacobs-university.de, w.nau@jacobs-university.de

Abstract

A supramolecular host–guest FRET pair based on a carboxyfluorescein-labelled cucurbit[7]uril (CB7-CF, as acceptor) and the fluorescent dye 4′,6-diamidino-2-phenylindole (DAPI, as donor) is developed for sensing of DNA. In comparison to the commercial DNA staining dye SYBR Green I, the new chemosensing ensemble offers dual-emission signals, which allows a linear ratiometric response over a wide concentration range.

This article is part of the themed collections: Cucurbiturils and related cavitands and 1st International Conference on Noncovalent Interactions

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Article information

DOI: https://doi.org/10.1039/C8CC09126A
Article type: Communication
Submitted: 16 nov 2018
Accepted: 10 dec 2018
First published: 10 dec 2018

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Chem. Commun., 2019,55, 671-674

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Ratiometric DNA sensing with a host–guest FRET pair

S. Zhang, K. I. Assaf, C. Huang, A. Hennig and W. M. Nau, Chem. Commun., 2019, 55, 671 DOI: 10.1039/C8CC09126A

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Chemical Communications

Ratiometric DNA sensing with a host–guest FRET pair†

Abstract

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Ratiometric DNA sensing with a host–guest FRET pair

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