A ratiometric fluorogenic probe for the real-time detection of SO32− in aqueous medium: application in a cellulose paper based device and potential to sense SO32− in mitochondria

Soham Samanta; Senjuti Halder; Poulomi Dey; Utsab Manna; Aiyagari Ramesh; Gopal Das

doi:10.1039/C7AN01368J

A ratiometric fluorogenic probe for the real-time detection of SO₃²⁻ in aqueous medium: application in a cellulose paper based device and potential to sense SO₃²⁻ in mitochondria†

Soham Samanta,^a Senjuti Halder,^a Poulomi Dey,^b Utsab Manna,^a Aiyagari Ramesh*^b and Gopal Das

*^a

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* Corresponding authors

^a Department of Chemistry, Indian Institute of Technology Guwahati, Guwahati 781039, India
E-mail: gdas@iitg.ernet.in
Fax: +91 361 2582349
Tel: +91 361 2582313

^b Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, India
E-mail: aramesh@iitg.ernet.in
Fax: +91 361 2582249
Tel: +91 361 2582205

Abstract

A new water soluble and fluorogenic probe (L) that can demonstrate a specific ratiometric detection of a SO₂ derivative (SO₃²⁻) in 100% aqueous medium and live cells has been designed and synthesized. The detection process can be visualized by the naked eye, as the orange-red fluorescence of L turns into a strong blue fluorescence upon interaction with SO₃²⁻. L displayed several beneficial attributes such as detection in complete aqueous medium, extremely fast response time along with high selectivity and sensitivity. The ratiometric sensing was attributed to the selective nucleophilic addition reaction of SO₃²⁻ with L. The probe was further used to develop a low cost microfluidic sensor device (μPAD). The probe was biocompatible and its potential to sense SO₃²⁻ in mitochondria was captured in live HeLa cells.

This article is part of the themed collections: Optical Biosensor Devices and Bioanalytical Sensors

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Article information

DOI: https://doi.org/10.1039/C7AN01368J
Article type: Paper
Submitted: 17 aug 2017
Accepted: 01 nov 2017
First published: 08 nov 2017

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Analyst, 2018,143, 250-257

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A ratiometric fluorogenic probe for the real-time detection of SO₃²⁻ in aqueous medium: application in a cellulose paper based device and potential to sense SO₃²⁻ in mitochondria

S. Samanta, S. Halder, P. Dey, U. Manna, A. Ramesh and G. Das, Analyst, 2018, 143, 250 DOI: 10.1039/C7AN01368J

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