Issue 16, 2016

Preparation of an aptamer based organic–inorganic hybrid monolithic column with gold nanoparticles as an intermediary for the enrichment of proteins

Abstract

A novel strategy for the preparation of an aptamer based organic–inorganic hybrid affinity monolithic column was developed successfully using gold nanoparticles (GNPs) as an intermediary for a sandwich structure to realize the functional modification of the surface of the monolithic matrix. This monolithic matrix was facilely pre-synthesized via one-step co-condensation. Due to the high surface-to-volume ratio of GNPs and the large specific surface area of the hybrid matrix, the average coverage density of aptamers on the hybrid monolith reached 342 pmol μL−1. With the combination of an aptamer based hybrid affinity monolithic column and enzymatic chromogenic assay, the quantitation and detection limits of thrombin were as low as 5 nM and 2 nM, respectively. These results indicated that the GNPs attached monolith provided a novel technique to immobilize aptamers on an organic–inorganic hybrid monolith and it could be used to achieve highly selective recognition and determination of trace proteins.

Graphical abstract: Preparation of an aptamer based organic–inorganic hybrid monolithic column with gold nanoparticles as an intermediary for the enrichment of proteins

Supplementary files

Article information

Article type
Paper
Submitted
25 apr 2016
Accepted
03 jún 2016
First published
03 jún 2016

Analyst, 2016,141, 4961-4967

Preparation of an aptamer based organic–inorganic hybrid monolithic column with gold nanoparticles as an intermediary for the enrichment of proteins

J. Zhao, Q. Zhu, L. Zhao, H. Lian and H. Chen, Analyst, 2016, 141, 4961 DOI: 10.1039/C6AN00957C

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