Issue 65, 2022

Selectivity and efficiency in the ligation of the pyrene:abasic base pair by T4 and PBCV-1 DNA ligases

Abstract

Remarkable selectivity was observed in the ligation of 5′-phosphate 1-pyrene nucleotide terminated strands across from an abasic lesion in a DNA-templated ligation reaction by two different ligases suggesting that pyrene-terminated strands could be used in abasic site detection. Increasing ATP concentration was critical to enhancing the selectivity for this base pair with T4 DNA ligase.

Graphical abstract: Selectivity and efficiency in the ligation of the pyrene:abasic base pair by T4 and PBCV-1 DNA ligases

Supplementary files

Article information

Article type
Communication
Submitted
31 máj 2022
Accepted
13 júl 2022
First published
18 júl 2022

Chem. Commun., 2022,58, 9072-9075

Selectivity and efficiency in the ligation of the pyrene:abasic base pair by T4 and PBCV-1 DNA ligases

H. Park and J. M. Gibbs, Chem. Commun., 2022, 58, 9072 DOI: 10.1039/D2CC03079A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements