Hongqian Chuab,
Tingming Caoab,
Guangming Daiab,
Bei Liud,
Huijuan Duanab,
Chengcheng Kongab,
Na Tianab,
Dailun Hou†
*c and
Zhaogang Sun†*ab
aTranslational Medicine Center, Beijing Chest Hospital, Capital Medical University, Beijing 101149, PR China. E-mail: sunzhaogang@bjxkyy.cn
bBeijing Key Laboratory in Drug Resistant Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, PR China
cDepartment of Radiology, Beijing Chest Hospital, Capital Medical University, Beijing, 101149, PR China. E-mail: houdailun@163.com
dSchool of Science, Minzu University of China, Beijing 100081, PR China
First published on 3rd November 2021
Upconversion nanoparticles (UCNPs) are a class of optical nanocrystals doped with lanthanide ions that offer great promise for applications in controllable tumor therapy. In recent years, UCNPs have become an important tool for studying the treatment of various malignant and nonmalignant cutaneous diseases. UCNPs convert near-infrared (NIR) radiation into shorter-wavelength visible and ultraviolet (UV) radiation, which is much better than conventional UV activated tumor therapy as strong UV-light can be damaging to healthy surrounding tissue. Moreover, UV light generally does not penetrate deeply into the skin, an issue that UCNPs can now address. However, the current studies are still in the early stage of research, with a long way to go before clinical implementation. In this paper, we systematically analysed recent advances in light-activated tumor therapy using functionalized UCNPs. We summarized the purpose and mechanism of UCNP-based photodynamic therapy (PDT), gene therapy, immunotherapy, chemo-therapy and integrated therapy. We believe the creation of functional materials based on UCNPs will offer superior performance and enable innovative applications, increasing the scope and opportunities for cancer therapy in the future.
To date, various nanoscale pharmaceutical carriers have been investigated in terms of possible cancer therapies, including liposomes, high molecular weight polymers, carbohydrates (e.g. carbon nanotubes, graphene oxide), mesoporous silica, magnetic nanoparticles, gold nanoparticles and other nanoparticles.7,8 Even though therapeutic drugs can be delivered by a nanoscale drug carrier to the tumor due to the phenomenon of local increased permeability and retention (EPR) at the tumor site, premature drug release can trigger organ damage and reduce the efficiency of drug release inside tumor cells, thus decreasing the therapeutic effectiveness,9,10 which significantly hindered clinical applications. The exploration of new nanoscale systems with targeted and controllable drug-release profiles is urgently needed to tackle the above-mentioned poor effectiveness and poor supply quality.11–14
Controlled activation or release of biomolecules is crucial for many different biological applications. Various methods were used to track the behaviour of biomolecules, and light-induced activation has gained popularity in recent decades. The key obstacle in this process is that photo-activated compounds mainly react to ultraviolet (UV) and not to visible or near-infrared (NIR) light. Upconversion nanoparticles (UCNPs) are a unique class of lanthanide-doped optical nanocrystals.15,16 UCNPs can transform two or more low-energy pump photons from the NIR to a higher-energy photon with a shorter wavelength,17–20 which has drawn considerable interest in many fields, including biomedicine. The mechanism of upconversion luminescence (UCL) has been identified and was developed for applications in biosensing, imaging and therapy, especially in oncology.21–24 Fig. 1 shows NaGdF4:Yb/Tm@NaGdF4, a typical example of a UCNP with a core–shell structure. These UCNPs exhibited characteristic UCL bands at 313, 363, 453, and 478 nm upon 980 nm CW laser excitation.25 Due to the unique optical properties of UCNPs, they can be designed and modified to achieve effective drug delivery and release guided by a specific stimulus. Several NIR-responsive drug delivery systems based on UCNPs have been successfully developed, offering great promise for effective cancer treatment using UCNPs as efficient NIR light transducers to activate therapeutics that react to visible or UV light. Here, we mainly discuss recent advances in functionalized UCNPs for light-activated tumor therapy.
In this review, we aim to provide a landscape of the strategies of using UCNPs for light-activated tumor therapy. We underline the four UCNPs-based tumor therapy methods and the agents that play key roles in material fabrication.
Fig. 2 (a) Scheme of Ce6 physically adsorbed on the surface of PEGylated UCNPs via hydrophobic interactions to form UCNP-Ce6 complex. (b) Representative photos of mice after various treatments indicated at the 6th day.7 |
Fig. 3 (a) The formation mechanism of NaYF4:Er/Yb/Gd@SiO2 (MB) in the water-in-oil reverse microemulsion system.59 (b) Schematic of the design of the versatile photosensitizer based on photon upconverting nanoparticles.58 |
UCNPs coated with porous silica have become an appealing drug delivery system that combines a light conversion property with a high loading capacity, enabling the physical encapsulation of payload molecules and achieving light-triggered drug release and activation.56,57 Zhang et al.58 as well as Shi et al.59 reported the first attempts to mechanically encapsulate PS molecules using silica-coated UCNPs for PDT (Fig. 3). The encapsulation was conducted in the solution in both cases, so that the negative charge and hydrophilic properties of the silicate layer together with the hydrophobic photosensitizer showed an unfavorable encapsulation effect, which resulted in a low efficiency of PS loading and leakage of PS from the particle surface.58 Another loading strategy developed for UCNP-based delivery systems is physical adsorption via electrostatic attraction.60–63
Recent advances in UCNPs involving co-loaded anticancer drugs (DOX) and chlorine Ce6 with high antitumor effectiveness have showed great promise in the manufacturing of bifunctional materials (pH- and ROS-responsive). As shown in (Fig. 4), on the surface of UCNPs@mSiO2, DOX and Ce6 were conjugated and prepared. The average size of UCNPs@mSiO2 after loading DOX and Ce6 was 85.63 ± 9.87 nm. The resulting UCNPs@mSiO2 had a small size (<100 nm) for further loading of drug and PS, which yields a photo-induced drug delivery system and may boost the cell uptake and activate Ce6, which regulated the release of DOX by 980 nm NIR irradiation.64
Fig. 4 (a) Schematics of the UCNPs and UCNPs@mSiO2. (b) Preparation and characterization of DOX/Ce6 conjugates and loading on the surface of UCNPs@mSiO2. (c) Schematic of the mechanism of the NP internalization and combined pH drug release and PDT effects under the NIR light exposure. (d) Relative viabilities of the cancer cell lines incubated for various times with different concentrations of UCNPs@mSiO2-DOX/Ce6 exposed to the 980 nm laser (0.45 W cm−2, 10 min).64 |
The authors also developed a mesoporous silica-coated UCNP system in which photochemically incorporated porphyrin PSs and NIR-responsive diarylethene (DAE) photochromic switches are loaded into nanopores. The UCNP core absorbs low-energy photons when irradiated with 980 nm NIR light. It transfers energy to the silica walls and efficiently produces 1O2. This 980 nm NIR light-sensitized behaviour can also be controlled remotely by 808 nm light irradiation. In the closed form of the DAE mounted in the nanopores, 1O2 generation is inhibited, while after irradiation with 808 nm NIR light, the DAE is transformed into an open shape, thus enhancing the 980 nm NIR production of 1O2 (Fig. 5). Also, the outer shell thickness of <3 nm further enhances the efficiency of resonance energy transfer between UCNPs and PSs. The NIR on-demand light-mediated nanoarchitecture activations has great advantages for the efficient delivery of an integrated photosensitive system in living cells that allows for in vitro and in vivo activation of PDT with efficient control of 1O2 release.65 The loading of PSs in the pores of mesoporous silica may enhance its stability in the complicated cell microenvironment compared to a solid silica sheet and contribute to a faster generation and release of 1O2 in the target tissue.
Fig. 5 (a) Schematic showing the synthesis procedure of UC@PS/C-DAE. (b) Schematic illustration of the 980 nm NIR light photosensitized UC@PS/C-DAE nanoconstruct, whose 1O2 generation capability can be remotely switched on by 808 nm NIR light irradiation. (c and d) Representative time-dependent in vivo fluorescence imaging of 4T1 tumor-bearing mice after exposure to different treatments. (e) The 4T1 tumor growth curves after different treatments. (f) Histological images of the tumor sections on day 14 post various treatments. The tissues were stained with H&E and TUNEL, respectively.65 |
The rapid growth of tumor cells requires large amounts of oxygen, leading to a hypoxic tumor microenvironment, which is an undesirable condition in the tumor that drastically reduces the efficacy of PDT.66–68 As shown in (Fig. 6), Liu et al. constructed new UCNPs named TPZ-UC/PS, which have a co-delivery capacity for PSs and the prodrug tirapazamine (TPZ) which can be activated and becomes extremely toxic under hypoxic conditions.69 This is an effective synergetic PDT/chemo-T, as TPZ remains active even under oxygen depletion conditions and induces cytotoxicity. This shows that chemo-T with TPZ can overcome the drawbacks of PDT in synergetic cancer treatment.
Fig. 6 (a) Fluorescence spectrum of UCNPs under 980 nm laser light excitation (red line) and UV/Vis absorption spectrum of SPCD (black line). (b) Formation of UCNPs coated with PS-doped dense silica. (c–e) Representative transmission electron micrographs of UCNPs, UCNP coated with dense silica, and UCNPs coated with dense and outer mesoporous silica shells (UC/PS). (f) Flow cytometric analyses of cell apoptosis treated with PBS, TPZ, UC/PS, or TPZ-UC/PS. Non-NIR: unirradiated cells; NIR: cells irradiated with 980 nm laser light.69 |
Fig. 7 (a) Schematic of covalent conjugation of NaYF4:Yb3+, Er3+ UCNPs, photosensitizer RB, and target molecule FA. (b) Viability of JAR cells (left) and NIH 3T3 fibroblasts (right) treated with UCNPs-PS of different concentrations with (solid) or without (open) 980 nm exposure.70 |
Xia et al. constructed a nanodevice named UCNPs-ZnPc, a highly efficient nanophotosensitizer for PDT, based on NIR light UCNPs and Zn(II)-phthalocyanine (ZnPc) PS.71 The high 1O2 production efficiency was achieved by enhancing the 660 nm upconversion emission of UCNPs through Yb3+ doping. The covalent linkage of UCNPs to ZnPc decreased the distance between the two and enhanced energy transfer. The high production of 1O2, as shown in in vivo tests in which the material was injected locally into the tumor site of mice, could achieve safe and effective PDT treatment. The healthy organs showed no pathological and inflammatory changes. The deficiency of the design is the requirement of intratumoral injection, which is not suitable for deep tumors and not a safe way of determining changes of normal organs.
In order to increase the efficacy of an NIR-light-activated PDT by attenuating the status of tumor hypoxia and synergistically reprogramming the populations of tumor-related macrophages, Ai et al. constructed a unique PUN nanodevice (UCNs-MnO2-Ce6-HA), with Ce6 loaded onto UCNPs, which was modified with manganese dioxide (MnO2) nanosheets and then coated with hyaluronic acid (HA) biopolymer (Fig. 8). The system was able to react with overproduced H2O2, which is degraded by the MnO2 nanosheets to produce a massive oxygen burst, and thus could significantly enhance PDT efficiency by 808 nm light irradiation. Furthermore, the bioinspired polymer HA could effectively reprogram the polarization of pro-tumor M2-type TAMs into anti-tumor M1-type macrophages to prevent tumor recurrence following PDT. Such promising results provided great opportunities for improved tumor ablation with NIR light-mediated PDT treatment by microtube attenuation and reprograming of tumor-related macrophages.72
Fig. 8 Illustration of NIR light-mediated PDT strategy for the enhanced cellular ablation in tumor microenvironment. (a) Design and synthesis of PUN. (b) Scheme of improved therapy by attenuating hypoxia status and reprogramming TAMs from M2 to M1 phenotype to inhibit the recurrence of tumor cells toward immunotherapy during the post-PDT period.72 |
Han's group designed a nanostructure using Nd3+ and Yb3+ as photosensitizers and Ho3+ as an active material.73 Since Ho3+ emits light in the wavelength range of 540 nm and 650 nm in which rose bengal (RB) can be activated, it was used as a photosensitizer (Fig. 9). Covalent conjugation of the UCNPs with RB (UCNPs-RB) was designed in this study. By treating cells with UCNPs-RB followed by 808 nm light irradiation, a minimum overheating effect was verified by comparing UCNPs-RB treated cells with 980 nm light irradiation and the improved PDT efficiency was confirmed in HeLa cells.
Fig. 9 (a) Schematic illustration of the synthetic step of 808 nm-activable UCNPs with RB. (b) Viability of HeLa cancer cells treated with different concentrations of UCNPs-RB with and without 808 nm laser irradiation (0.67 W cm−2).73 |
Recently, Shi et al. designed a near infrared-driven PDT platform based on photosynthetic cyanobacterial cells hybridized with photosensitizer RB-loaded UCNPs, named UR-Cyan cells. Upon 980 nm NIR light irradiation, UR-Cyan cells showed strong oxygen production and further enhanced subsequent singlet oxygen generation by the photosensitizer, resulting in enhanced and sustainable PDT efficacy against tumor cells/tissues.74 Compared to cell membranes and other cytoplasmic organelles, the nucleus is highly susceptible to photodynamic damage due to its high sensitivity to PDT.75 Nucleus-based PDT can cause severe DNA damage and inactivation of intranuclear enzymes. Cell nucleus-based photodynamic therapy is a highly effective method for cancer treatment, but it is still challenging to design nucleus-targeting photosensitizers. Zhang et al. proposed a “one treatment, multiple irradiations” strategy to achieve nucleus-based photodynamic therapy based on RB-loaded MSN-coated UCNPs with amino-groups on the surface (UCNP/RB@mSiO2-NH2 NPs).76 UCNP/RB@mSiO2-NH2 NPs could be specifically accumulated in the acidic lysosomes due to their amino group-decorated surface and the ROS produced by RB could effectively destroy lysosomes after 980 nm NIR light irradiation. Subsequently, UCNP/RB@mSiO2-NH2 NPs were released from the lysosomes and were transferred into the cell nucleus, where a second round of 980 nm light irradiation achieved nucleus-based photodynamic therapy (Fig. 10).
Fig. 10 (a) Schematic illustrations of the preparation of UCNP/RB@mSiO2-NH2 NPs and their application in the nucleus-based PDT of cancer cells based on the “one treatment, multiple irradiations” strategy. (b) CLSM images of MCF-7 cells treated as indicated and then double-stained with calcein AM and propidium iodide (PI). Scale bars, 50 μm.76 |
We summarized the UCNP based PDT triggered by NIR light in Table 1. Despite its stable binding ability, the UCNP based PDT effect was still unsatisfactory, and this was mainly due to its relatively low conversion efficiency and weak PS loading, which further limits its application in in vivo anti-tumor studies.
PSs | Composite nanostructures | NIR laser (nm) | Laser dose (W cm−2) | Ref. |
---|---|---|---|---|
Ce6 | UCNPs-Ce6 | 980 | 0.5 | 7 |
UCNPs-Ce6 | 980 | 0.3 | 77 | |
UCNPs-Ce6-siRNA | 980 | 0.8 | 78 | |
UCNP-Ce6-sgc8 | 980 | 0.6 | 79 | |
UCNP@2 × Ce6-DMMA-PEG | 980 | 0.5 | 80 | |
Porphyrin | UCNP-PEG-FA/PC70 | 980 | 0.8 | 81 |
UCNP-TMPyP4-G4-aptamer | 980 | 0.5 | 82 | |
UCNP-PJ | 980 | 1.0 | 83 | |
RB | UCNPs@RB@RGDRBC@ICG | 980 | 1.5 | 84 |
UCNPs-RB | 808 | 1.15 | 85 | |
MUCNPs-800CW/Cy3/RB/Pep-QSY7 | 808 | 1.5 | 86 | |
MOF | MOF-UCNP | 980 | 15.9 | 87 |
UCMTs | 808 | 1.0 | 88 | |
CR@MUP | 808 | 1.0 | 89 |
Xiang et al. were the first to develop antigen-loaded UCNPs for DC-based immunotherapy, and the system could be used to track dendritic cells in vivo using upconversion luminescence imaging.115 Our team has developed an UCNPs-based immunotherapy device that enables remote control of antitumor immunity with NIR light both in vitro and in vivo. As shown in (Fig. 11), the immunotherapy device was constructed using UCNPs, immunotherapeutic CpG oligonucleotides and CpG complementary ssDNA containing photocleavable (PC) bonds that can be cleaved by UV light transduced by UCNPs, thus achieved remotely controlled immune triggering at the right time. The average size of the UC/PCpG is about 45 nm, which promotes the EPR effect.116 However, one deficiency of this study is that the material reaches the tumor site through passive targeting, which is less effective than active targeting.
Fig. 11 (a) Schematic of the design of photoactivatable immunodevice through the integration of UCNPs with the UV light-responsive PCpG. UCNPs upconvert NIR light into UV light locally, thus liberate CpG ODN from PCpG to achieve immunotherapy effects; (b) illustration of the photoactivatable immunodevice, PCpG/UCs, for spatially selective triggering of immunoactivity through NIR light irradiation. In contrast to traditional CpG delivery system (CpG/UCs), PCpG/UCs is amenable to personalizing the antitumor modality with reduced systemic toxicity; (c) representative pictures of the tumors; (d) Kaplan–Meier survival curves of mice in the different treatment groups; (e and f) flow cytometric analysis of T cell populations in tumors from different treatment groups, showing percentages of total live cells.116 |
In recent years, a number of studies concentrated on immunotherapy combined with other treatments for cancer therapy.117 In addition to being used as individual therapy, research has shed light on several potential alternative therapies, such as combining the sensitization effects of radiotherapy, chemotherapy, photothermal therapy (PTT), and immunotherapy, which show additive or synergistic benefits. PDT is an anticancer therapy that can induce immunogenic cell death and activate an adaptive immune response against tumor-associated antigens.118–120 Many studies investigated synergistic treatments based on PDT and immunotherapy to trigger antitumor immunity.121–123 Xu et al. conjugated the photosensitizer Ce6 and the Toll-like receptor 7 agonist R837 to UCNPs, obtaining multitasking UCNP-Ce6-R837 nanoparticles that could achieve effective PDT under NIR irradiation (Fig. 12). As expected, PDT triggered the release of tumor-associated antigens, while the R837-activated TLR7 pathway could induce strong systemic immunological responses and could be further enhanced by CTLA-4 blockade.124 The greatest benefit of the system is that the nanodevice could prevent tumor recurrence via the immune memory effect.
Fig. 12 (a) Schematic illustration of NIR triggered PDT with multitasking UCNPs in combination with checkpoint blockade for immunotherapy of cancer. (b) Growth curves for primary tumors (b) and distant tumors (c) on mice after various treatments indicated.124 |
The combination of different therapy methods has become a smart strategy for cancer treatment. Recently, Li's group designed a composite nanomaterial that combined PDT, chemotherapy and immunotherapy together to fight hypoxic tumors.125 They combined metal–organic frameworks (MOFs) and UCNPs to construct a heterostructure, which was then loaded with the hypoxia-activated prodrug tirapazamine (TPZ), thus combining NIR light-triggered PDT and chemotherapy against hypoxia tumors. Moreover, the integration of the composite nanoparticles with anti-programmed death ligand-1 (α-PD-L1) treatment completely inhibited distant tumors by inducing infiltration by cytotoxic T cells (Fig. 13).
Fig. 13 (a) Schematic illustration of the synthesis of UCDs and UCSs through the conditional surface engineering of UCNPs. (b) Schematic illustration of the structure of TPZ/UCSs and their application for tumor treatment through the combination of NIR light-triggered PDT and hypoxia-activated chemotherapy with immunotherapy. (c) Schematic illustration of NIR light-triggered combinational therapy. Growth curves of (d) primary tumors and (e) distant tumors in CT26 tumor-bearing mice after different treatments. (f and g) Photos and average weights of primary tumors (left column) and distant tumors (right column) collected from mice 14 days after treatments.125 |
Even though PDT and chemotherapy show clinical promise in destroying orthotopic tumors, they still show insufficient abscopal effects against metastases. The combination of PDT, chemotherapy and immunotherapy promises to exert a strong effect against distant metastases. Du et al. reported a new nanosystem that combines an anti-CD73 antibody and chemo-PDT to synergistically amplify the abscopal effects of T cell-mediated antitumor immunity.126 The cancer cell membrane (CM)-cloaked UCNPs were integrated with RB and the ROS-sensitive polymer polyethylene glycol-thioketal-doxorubicin (PEG-TK-DOX), followed by their application for NIR-triggered chemo-PDT. The CM coating promoted the active targeting of the nanoparticles to the tumor and aided immune escape from macrophages. Chemo-PDT presents strong synergistic antitumor efficacy and causes immunogenic cell death (ICD), leading to tumor-specific immunity. The anti-CD73 antibody prevents immunosuppression by tumors and acts as a sufficient immune checkpoint blockade when combined with ICD-elicited tumor therapies (Fig. 14). Combined therapy, especially treatment approaches that incorporate immunotherapy, is a promising strategy for curing metastatic cancer in preclinical research. Chen et al. designed a UCNP-based antigen-capturing nanoplatform to synergize phototherapies and immunotherapy.127 The nanoplatform is constructed via self-assembly of DSPE-PEG-maleimide and indocyanine green (ICG) onto UCNPs, followed by loading with the photosensitizer RB. ICG significantly enhances the efficiency of RB-based PDT upon NIR light activation, simultaneously achieving selective PTT. Most importantly, maleimide could capture and keep tumor associated antigens in situ, which were derived from phototherapy-treated tumor cells, and further enhance the uptake and presentation of tumor antigens. The combined PDT, PTT, and immunological effects induces a tumor-specific immune response. When combined with anti-CTLA-4, the nanoplatform showed a strong ability to destroy primary tumors and inhibit untreated distant tumors. Overall, combined immunotherapy provides a promising approach for the treatment of metastatic cancers.128
Fig. 14 Schematic illustration of the synthesis process of cancer membranes-camouflaged RB, DOX co-loaded UCNPs (CM@UCNP-RB/PTD) and the application in chemo-PDT combination therapy with CD73 blockade to enhance synergistic antitumor immunity in combating metastatic tumor.126 |
Fig. 15 Surface modification of UCNPs for gene delivery.133 |
A pioneering investigation of UCNPs for nucleic acids delivery was conducted by Zhang's group.134 In their study, an anti-Her2 antibody was conjugated to amino-modified UCNPs for targeted delivery, and siRNAs were attached to the surface of UCNP-antiHer2 through electrostatic interactions. Material uptake by Her2-overexpressing SK-BR-3 cells was successfully confirmed, shown by the downregulated expression of the luciferase reporter gene. Subsequently, surface improvements to the abovementioned delivery system were further applied to improve the gene loading efficiency and increase the targeted distribution.135–137 Recently, it was also demonstrated that UCNPs can be used to precisely regulate gene expression in tumor cells transplanted into adult zebrafish.138 Similar success was also achieved using the NIR-to-UV upconversion process of silica-coated NaYF4:Yb,Tm nanocrystals to temporally and spatially silence the expression of target genes.139 This ability to manipulate gene expression has not only practical significance for the development of therapies, but also for basic research on signal transduction. All of these applications are beyond the scope of conventional gene delivery methods.
When a foreign gene is delivered into cells by the carrier, one important obstacle is that the sequestration of endosomal vesicles may lead to material degradation or exocytotic transport out of the cells.140 Zhang's group used the photochemical internalization (PCI) method to enhance the endosomal escape of the delivered genes and boost the therapeutic effect. In said study, UCNPs were co-loaded with TPPS2a (PSs) and photo-morpholino (anti-STAT3, nucleic analogous for gene knockdown).141 Under NIR excitation, TPPS2a was triggered by UCNP to generate ROS to locally disrupt the endosomal vesicle walls and trigger the release of the particles. At the same time, the UV light emitted by UCNPs cleaved the photo-morpholino senses strand and released the antisense morpholino, which further knocked down STAT3 gene expression (Fig. 16a). Compared to the control group, effective gene knockdown was achieved without facilitating endosomal escape demonstrating the excellent clinical application potential of this platform.
Fig. 16 Scheme representations of gene therapy with DNA-functionalized UCNPs. (a) Gene knockdown with UCNP-based photoactive delivery platform.141 (b) The functionalization of UCNPs, co-loading with Ce6 and siRNA, and then the combined PDT and gene therapy delivered by UCNPs.78 (c) Synthesis of UCNOs (UCNP-PEIRB-PEISeSe/siRNA-R8-HA), and (d) sequential responsive decompose of UCNOs and NIR boosted intracellular siRNA release and therapy.144 |
RNA interference (RNAi) is considered a promising tool for cancer treatment.142 The high efficacy of siRNA is currently constrained by limitations of its delivery methods. Hence, improvements to the specificity and effectiveness of existing delivery systems are required before exploring clinical applications. Wang et al. engineered and synthesized polymer-coated UCNPs loaded with Ce6 and Plk1 oncogene-targeting siRNA (Fig. 16b). The NIR laser-mediated PDT and siRNA delivery induced significant cancer cell apoptosis.78 Such a combination of PDT and gene therapy greatly enhances the cancer cell killing effects and has relatively few side effects compared to conventional multimodal therapeutic systems focused on chemotherapy.
A key challenge in siRNA delivery is how to prevent the biomolecules from being degraded by enzymes in complicated physiological environments and how to trigger efficient on-demand release.143 To solve this problem, He et al. designed a composite nanomaterial which is composed of UCNPs coated with PEI conjugated RB, and further coated with 1O2 sensitive diselenide linked PEI, which was modified with therapeutic siRNA loading and cell-penetrating peptide R8, as well as an outer layer coated with hyaluronic acid (HA) (Fig. 16c).144 NIR irradiation increased the decomposition of composite nanomaterials and induced the rapid and efficient release of siRNA, which effectively increased the in vitro gene silencing efficiency and in vivo tumor suppression (Fig. 16d). The sequentially sensitive UCNPs may have promising potential applications in precision medicine.
Recently, Zhang et al. developed a photo-degradable nano-capsule for efficient NIR-modulated siRNA delivery. The photo-degradable nano-capsules are based on core–shell UCNPs coated with an MSN layer for loading of photosensitizer HA and siRNA against polo-like kinase 1 (PLK1), covalently bound with a thin membrane of polyethylene glycol (PEG) via a synthesized photocleavable linker (PhL).145 Upon 980 nm irradiation, the UCNPs produce UV emissions to break the PhL and detach the PEG membrane to release siRNA (Fig. 17a). ROS allows the cargo to escape the endosomes and increases the gene silencing performance, suppressing cell proliferation in vitro and tumor growth in vivo (Fig. 17b). The photo-degradable membrane-bound nano-capsules may have promising applications in precision medicine.
Fig. 17 Schematic illustrations of (a) synthesis of UCNP nano-capsules, and (b) folate receptor-mediated cellular uptake and NIR modulated intracellular siRNA delivery and therapy. (c) Demonstration of therapeutic efficiency with representative photos of xenograft tumors in mice on day 14 and (d) change of tumor volume as a function of time after treatment with the indicated materials.145 |
Lin et al. designed layer by layer UCNPs for monitoring and delivery of MDR1 gene-silencing siRNA (MDR1-siRNA) to enhance the effectiveness of chemotherapy by silencing the MDR1 gene and increase the drug sensitivity of ovarian cancer cells.146 PAA and PEI were coated onto the surface of UCNOs and then loaded MDR1-siRNA by electrostatic adsorption (Fig. 18). The UCNP-based carrier increased the cellular uptake of MDR1-siRNA, protected them against nuclease-mediated degradation, and facilitated endosomal escape for effective MDR gene silencing.
Fig. 18 (a) Schematic illustration for the preparation of layer-by-layer assembled UCNP/PAA/PEI/siRNA complex. (b) Western blot for the detection of P-gp expression in response to OVCAR8TR cells with different treatments. Lanes 1–4: untreated, UCNP/PAA/PEI, free MDR1-siRNA, and UCNP/PAA/PEI/MDR1-siRNA complex. (c) Quantitative analysis of P-gp expression. (d) OVCAR8TR cells viability to different treatments.146 |
Due to their capacity to specifically bind to targets and deliver a large amount of therapeutic drugs, nanoparticles have attracted wide attention in medicine. Such materials could be adapted for cancer chemotherapy and various other diseases by making therapeutic delivery into diseased cells more efficient while lowering the exposure of healthy tissue to toxic side effects.155,156 Accordingly, UCNPs have been studied as nanocarriers for anticancer drugs using appropriate surface modification and functionalization. However, pre-release of anticancer drugs before the particles arrived at the target might lead to severe side effects and reduce their effectiveness. To solve this problem, there is a great need to develop UCNP-based targeted drug delivery systems, which can be used as contrast agents for bioimaging and on-demand drug release. However, few UCNP-based single chemotherapy studies were reported, and most studies were focused on the combination of UCNP-based chemotherapy with other therapies, such as PDT or photothermal therapy (PTT).
Chen et al. developed a multifunctional, dual-drug carrier platform based on UCNPs@SiO2 loaded doxorubicin (DOX), poly(ε-caprolactone) and gelatin loaded with an anti-inflammatory drug together with indomethacin to form nanofibrous fabrics.157 These nanofibrous fabrics can be implanted at the tumor site to exert orthotopic chemotherapy by controlled release of DOX from mesoporous SiO2 (Fig. 19a). This nano-platform showed an excellent antitumor effect in situ (Fig. 19b–d), and the nanofibers can also be used for fluorescence/magnetic resonance dual-model imaging (Fig. 19a).
Fig. 19 (a) Schematic illustration for multifunctional MC/UCNPS/DOX nanofibers for synchronous up-conversion luminescence/magnetic resonance (UCL/MR) imaging and therapy of tumor in vivo. (b–d) The anti-tumor effects of the nanofibers in vivo.157 |
In addition to direct delivery of chemotherapy drugs to the tumor site, UCNPs can be designed to construct TME responsive drug release systems. Li et al. designed a pH-responsive nanoparticle WP5⊃1-UCNPs (Fig. 20a). Carboxylatebased pillar arene (WP5) and 15-carboxy-N,N,N-trialkylpentadecan-1-ammonium bromide (1)-functionalized UCNPs can be decomposed by the acidic lysosome to release DOX loaded onto the UCNPs@SiO2.158 Cytotoxicity experiments have shown the outstanding biocompatibility of WP5⊃1-UCNPs without DOX loading, and that the DOX-WP5⊃1-UCNPs nanosystem has been proved to effectively kill HeLa cells (Fig. 20b).
Fig. 20 (a) Schematic illustration of the synthesis of DOX-WP5⊃1-UCNPs and the UCNPs based TME responsive drug-releasing nanosystem. (b) Fluorescence microscopy images of calcein-AM/ethidium homodimer-1-stained HeLa cells (green represents living cells, red represents dead cells) incubated with the concentration range of 12.5–200 μg mL−1 of WP5⊃1-UCNPs or DOX-WP5⊃1-UCNPs, or DOX.158 |
Relying on chemotherapy alone cannot always achieve the desired therapeutic effects. Almost all of the UCNP-based chemotherapy platforms were designed for combination with other therapies, such as PDT, PTT, immunotherapy and so on.159–161 Stimuli-responsive nanoparticles with multiple therapeutic functions are highly attractive for effective tumor treatment.162–166 Zhao et al. designed a composite nanoparticle named MPPa/UCNP-DEVD-DOX/cRGD, which was formulated by the assembly of UCNP, caspase-3 responsive DOX, a photosensitizer (pyropheophorbide-a methyl ester, MPPa) and tumor-targeting cRGD-PEG-DSPE to afford multifunctional CFUNs (Fig. 21a).167 After the particles were delivered into the cells, NIR irradiation was applied and the UCNPs emitted visible light inducing MPPa to produce ROS for PDT. Caspase-3 then triggered the release of DOX within tumor cells, thus accomplishing NIR-triggered PDT and cascade chemotherapy. The CFUN system enables the spatiotemporal control of NIR-triggered cascade therapeutic activation and tumor inhibition due to consecutive PDT and chemotherapy (Fig. 21b–e).
Fig. 21 (a) Schematic illustration of the synthesis of MPPa/UCNP-DEVD-DOX/cRGD and the mechanism of anti-tumor effect of the particles. (b) Relative volume of the tumor. (c) Representative pictures of the tumors. (d) Body weight of each mouse. (e) Histological assessments of tissues using H&E staining in the different treatment groups.167 |
Recently, Xu et al. designed a composite nanosystem through the incorporation of copper sulfide (CuS) nanosheets with strong negative charges into mesoporous silica UCNPs (Fig. 22a).168 After DOX loading, synergistic treatment via PTT, PDT and chemotherapy can be accomplished. The nanocomposites demonstrated excellent anti-tumor efficacy under 808 nm light irradiation, overcoming the low efficiency and limited penetration depth of traditional UV light (Fig. 22b–f).
Fig. 22 (a) Schematic illustration of the synthesis of B/USCs-PEG-DOX and the release of loaded DOX. (b) The mechanism of anti-tumor effect of the particles. (c–f) The antitumor effects of the composite nanosystem.168 |
Despite remarkable progresses in nucleic acid-targeted tumor therapy, there are few reports on intracellular RNA and nuclear DNA-dual targeted cancer treatments via a single nano-delivery system.169 Ma et al. designed an upconversion nanoplatform cis-platinum pro-drug (DSP) and cytotoxic protein ribonuclease A (RNase A) dual-therapeutic agent-loaded large-pore mesoporous silica-coated β-NaYF4:20%Yb,2%Er@β-NaGdF4 upconversion nanoplatform for chemo-protein combination therapy. The nanosystem can not only transport cytotoxic protein molecules and cis-platin pro-drugs into tumor cells to induce intracellular RNA degradation-mediated and nuclear DNA-targeted killing of cancer cells, but also achieve upconversion luminescence (UCL) and magnetic resonance (MR) dual-mode bioimaging (Fig. 23).169 Moreover, the synergetic treatment via chemotherapy and protein therapy exhibits a better therapeutic effect in vivo compared with chemotherapy or protein therapy alone. This relatively simple RNA and DNA-dual-targeted nanosystem will open new avenues for cancer therapy.
Fig. 23 Schematic illustration of UCSPtR for UCL/MR dual-mode bioimaging and chemo/protein combined therapy. Quadrivalent cis-platinum prodrugs, DSP and cytotoxic protein RNase A were loaded into large-pore mesoporous silica-coated β-NaYF4:20%Yb,2%Er@β-NaGdF4 (UCS) nanoparticles to obtain UCSPtR nanocomposites. Once internalized, this nanoplatform can effectively release cytotoxic protein and DSP and thus induce intracellular RNA degradation-mediated and nuclear DNA-targeted killings of cancer cells, respectively, to achieve collaborative treatments of chemotherapy and protein therapy. Furthermore, upconversion luminescence (UCL) and magnetic resonance (MR) dual-mode bioimaging can be achieved simultaneously.169 |
Chemotherapy remains the gold standard for the treatment of non-resectable tumors, but there still remains the problem of the systematic toxicity of the drugs. Even though a lot of progress has been made, we still need to explore more sophisticated nanosystems to solve this pressing problem.
Yu et al. carried out a systematic study to compare the in vivo biodistribution, excretion, and biosafety of PEI modified NaYF4:Yb,Er (PEI@UCNPs) among three different exposure routes (IV, IP, and IG).173 A variety of methods, including inductive coupling plasma mass spectrometry (IPC-SM), histology, body weight and biochemical analysis were used to assess the biodistribution, excretion and biosafety of UCNPs, and no obvious toxicity of UCNPs was observed in mice exposed through either of the tested administration routes. Wang et al. designed an experiment to evaluate the effects of LaF3:Yb,Er UCNPs on zebrafish embryos.174 The results showed that LaF3:Yb,Er UCNPs did not exhibit obvious toxicity to zebrafish embryos at a relative low dose (<100 mg L−1) but exhibited chronic toxicity at relatively high does (>200 mg L−1) in vivo, resulting in malformations and a reduced hatching rate, as well as impeding embryonic and larval development (Fig. 24). Xiong et al. explored the chronic toxicity of PAA modified UCNPs in mice exposed up to 115 days.175 PAA-UCNPs did not exhibit overt toxicity to mice and the studies indicate that PAA-UNCPs may probably be used long-term for targeted imaging and therapeutic studies in vivo. We also investigated the toxicity of UCNPs to mice by tail vein injection.25 The pathology of normal organs (heart, liver, spleen, lungs and kidneys) showed no change compared with the control group, and there were also no changes in the serum levels of cytokines, alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The study indicated that UCNPs have low systematic toxicity and are suitable for in vivo applications. Although several studies have demonstrated the short-term safety of UCNPs in vitro or in vivo, their long-term chronic effects and bio accumulation potential are poorly understood.176,177
Fig. 24 Phenotypic changes of zebrafish embryos at 48 hpf. (a1, a2) Control group. (b1–b3) UCNPs <200 μg mL−1 groups. (c1–c5) UCNPs 200–400 μg mL−1 groups. Abbreviations: b, brain; e, eye; n, notochord; t, tail; ys, yolk sac; bs, bent spine; tm, tail malformation; oe, ocular edema; pe, pericardial edema; oy, opaque yolk; yes, yolk sac edema; and ynd, yolk not depleted.174 |
(i) Rational combination of therapeutic modalities: the current development of UCNP-based nanocarriers aims to create a single dose magic bullet combined with different treatments for cancer therapy. It is therefore important to boost the therapeutic efficacy at a lower dose while promoting a biocompatible carrier material and to decrease toxic side effects. Although some combinations were successfully introduced and had good results as planned, UCNP-based combined cancer treatment is still in the early stages. In addition, the random combination of therapeutic strategies not only leads to challenges in achieving the above-noted goal of synergistic cancer therapy, but may also have unforeseen and unintended harmful consequences. In addition, the unique spatiotemporal form of each monotherapy should be considered to target cancer when combining monotherapies for synergistic therapy.
(ii) The key potential risks in the clinical application of UCNPs are their systemic toxicity, the complexity of clearance and long-term effects on the human body. Furthermore, the relationship between the immune system, its interference with the reproductive system and its effect on the next generation are still uncertain. More systematic studies are also required for safety assessment before these particles are used in the clinic.
Footnote |
† These authors contributed equally to this work. |
This journal is © The Royal Society of Chemistry 2021 |