Issue 10, 2015

Engineering reprogrammable RNA-binding proteins for study and manipulation of the transcriptome

Abstract

With the expanding interest in RNA biology, interest in artificial RNA-binding proteins (RBPs) is likewise increasing. RBPs can be designed in a modular fashion, whereby effector and RNA-binding domains are combined in chimeric proteins that exhibit both functions and can be applied for regulation of a broad range of biological processes. The elucidation of the RNA recognition code for Pumilio and fem-3 mRNA-binding factor (PUF) homology proteins allowed engineering of artificial RBPs for targeting endogenous mRNAs. In this review, we will focus on the recent advances in elucidating and reprogramming PUF domain specificity, update on several promising applications of PUF-based designer RBPs, and discuss some other domains that hold the potential to be used as the RNA-binding scaffolds for designer RBP engineering.

Graphical abstract: Engineering reprogrammable RNA-binding proteins for study and manipulation of the transcriptome

Article information

Article type
Review Article
Submitted
23 apr 2015
Accepted
06 júl 2015
First published
06 júl 2015

Mol. BioSyst., 2015,11, 2658-2665

Author version available

Engineering reprogrammable RNA-binding proteins for study and manipulation of the transcriptome

Z. Abil and H. Zhao, Mol. BioSyst., 2015, 11, 2658 DOI: 10.1039/C5MB00289C

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