Issue 8, 2011

Iodination of proteins, proteomes and antibodies with potassium triodide for LA-ICP-MS based proteomic analyses

Abstract

A fast and mild method for iodine labelling of proteins is presented which is specifically designed and optimized for laser ablation (LA-)ICP-MS based proteomics. Single proteins (lysozyme, bovine serum albumin, cytochrome c and β-casein), whole proteomes (microsomal proteome of rats) and antibodies (anti-bovine casein, anti-bovine serum albumin) can be efficiently iodinated by means of potassium triiodide with minimal losses of antigen properties and antibody binding to iodinated proteins. A comparison with iodination by use of IODO-Beads is presented and it is shown that triiodide labelling is a fast, cheap and less laborious alternative without compromising the analytical figures of merit.

Graphical abstract: Iodination of proteins, proteomes and antibodies with potassium triodide for LA-ICP-MS based proteomic analyses

Supplementary files

Article information

Article type
Paper
Submitted
09 mar 2011
Accepted
18 máj 2011
First published
10 jún 2011

J. Anal. At. Spectrom., 2011,26, 1610-1618

Iodination of proteins, proteomes and antibodies with potassium triodide for LA-ICP-MS based proteomic analyses

L. Waentig, N. Jakubowski, H. Hayen and P. H. Roos, J. Anal. At. Spectrom., 2011, 26, 1610 DOI: 10.1039/C1JA10090D

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