Issue 38, 2011
From the journal:

Chemical Communications

Glycosphingolipid synthesis employing a combination of recombinant glycosyltransferases and an endoglycoceramidase glycosynthase†‡

Jamie R. Rich,a   Anna-Maria Cunningham,b   Michel Gilbertb  and  Stephen G. Withers*a  

* Corresponding authors

a Department of Chemistry and Centre for High-Throughput Biology, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia, Canada
E-mail: withers@chem.ubc.ca
Fax: 604 822 8869
Tel: 604 822 3402

b Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada

Abstract

Glycosynthase mutants of Rhodococcus sp. endo-glycoceramidase II efficiently synthesize complex glycosphingolipids. Glycosyl fluoride donors may be assembled via sequential glycosyltransferase-catalysed glycosylation of lactosyl fluoride. Alternatively, lactosyl fluoride may be coupled to sphingosine prior to subsequent glycosylation steps.

Graphical abstract: Glycosphingolipid synthesis employing a combination of recombinant glycosyltransferases and an endoglycoceramidase glycosynthase

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C1CC13885E
Article type
Communication
Submitted
30 jún 2011
Accepted
16 aug 2011
First published
30 aug 2011

Chem. Commun., 2011,47, 10806-10808

Permissions

Request permissions

Glycosphingolipid synthesis employing a combination of recombinant glycosyltransferases and an endoglycoceramidase glycosynthase

J. R. Rich, A. Cunningham, M. Gilbert and S. G. Withers, Chem. Commun., 2011, 47, 10806 DOI: 10.1039/C1CC13885E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements