A fluorene-conjugated diazirine is described, for which two-photon activation is demonstrated for the first time.
Linear diazirines can produce relatively long-lived diazoalkanes which are also capable of labeling proteins whereas cyclobutanediazirines are proposed to give minimal diazo product and labeling occurs predominantly via a carbene mechanism.
A bifunctional reagent containing a diazirine carbene precursor and a benzyl bromide electrophile can be used to photofunctionalize Kevlar and other inert polymers, providing sites for covalent attachment of dyes.
A novel, divergent radical cyclization of homoallylic diazirines for the synthesis of pyrrolines and fused diaziridines is described.
Photoaffinity labelling tool compounds were synthesised to investigate the allosteric binding on the DENV protease, elucidating the allosteric pocket.