A highly efficient aminoacyl tRNA synthetase (G1PylRSMIFAF) has been developed to produce MeHis-containing proteins. High protein titres can be achieved with low ncAA concentrations (0.1 mM) enabling more economical production of MeHis-containing enzymes.
Genetic code expansion (GCE) technology has been optimized, applied, and validated for single-molecule tracking (SMT) of intracellularly dye-labelled proteins in a fully re-coded E. coli strain.
We discuss recent advances in the fluorescent labeling of specific proteins in cells and its applications for studying protein-associated biological processes.
The genetic code expansion technique was employed to understand functions of the cell-surface receptor.
For living HeLa cells, we report a small and minimally-invasive Raman reporter (about 2 aa and <1 kDa), which can be site-specifically introduced into proteins by genetic codon expansion combined with tetrazine ligation.