Our best fluorogenic probe enables live-cell imaging with high-contrast and no need for washing steps.
Click’n lock describes a reaction system capable of switching on-demand from reversible to irreversible click reactions and is based on the chemoselective tetrazine–thiol exchange. Here, click’n lock is applied for dynamic modifications of peptides.
Evaluation of tetrazine-modulated fluorogenic Cy3 probes revealed that internal conversion-based quenching of fluorescence results in better fluorogenic performances even in spectral ranges where tetrazines have diminished modulation power.
Tetrazine-functionalised lanthanide chelates and their catalyst-free ‘click’ reactions with a trans-cyclooctene-rhodamine fluorophore are reported. These probes were delivered across the blood–brain barrier using focused ultrasound and microbubbles.
Genetic code expansion (GCE) technology has been optimized, applied, and validated for single-molecule tracking (SMT) of intracellularly dye-labelled proteins in a fully re-coded E. coli strain.