We analyzed the plasmid-mediated transfer frequency between Gram-negative and Gram-positive bacteria at different GO concentrations to unveil its impact on conjugative transfer of ARGs.
S-PSNPs facilitated conjugative transfer frequency of ARGs between E. coli strains than L-PSNPs, and the mechanisms include SOS response, membrane permeability and altered related gene expression.
The transfer of antibiotic resistance genes was influenced by the concentration and surface charge of nanoplastics. This was due to a combination of the promotion effect of ROS and the inhibition caused by nanoplastic agglomeration.
Traditional stormwater infrastructure facilitates the transport of antibiotic resistance genes while green stormwater infrastructure may capture the genes and promote horizontal gene transfer.
Our findings elucidate how surface characteristics drive ARG dissemination risks and provide mechanistic insights, informing strategies to mitigate antibiotic resistance in contaminated environments.