OoTrap: enhancing oocyte collection and maturation with a field-deployable fluidic device

Abstract

Assisted reproductive technologies (ART) are pivotal for contemporary reproductive medicine and species conservation. However, the manual handling required in these processes introduces stress that can compromise oocyte and embryo quality. This study introduces OoTrap, a novel fluidic device designed to streamline ART workflows by facilitating the capture and maturation of oocytes in a compact unit. The device also reintroduces mechanical forces similar to those in the in vivo environment, which are often missing in conventional systems. OoTrap operates in both static and perfusion-based modes, offering flexibility and optimal conditions for oocyte maturation. Notably, OoTrap achieved higher in vitro maturation (IVM) rates under perfusion, produced oocytes with fewer chromosomal abnormalities, and maintained spindle morphology integrity. The incorporation of a heating system and a 3D-printed syringe pump enabled IVM outside the incubator, making OoTrap suitable for field applications. The results highlight the potential of OoTrap to enhance ART outcomes by reducing manual handling, providing a controlled microenvironment, and offering a practical solution for field-based ART applications.