Themed collection Biocatalysis

30 items
Front/Back Matter

Poster list

From the themed collection: Biocatalysis
Front/Back Matter

List of participants

From the themed collection: Biocatalysis
Open Access Paper

Spiers Memorial Lecture: Engineering biocatalysts

Enzymes are being engineered to catalyze chemical reactions for many practical applications in chemistry and biotechnology.

Graphical abstract: Spiers Memorial Lecture: Engineering biocatalysts
From the themed collection: Biocatalysis
Paper

Indigo production identifies hotspots in cytochrome P450 BM3 for diversifying aromatic hydroxylation

Indigo (+) and indigo (−) single variants and a combinatorial library, with mutations that enable the blue phenotype, were screened for their ability to hydroxylate a panel of 12 aromatic compounds using the 4-aminoantipyrine colorimetric assay.

Graphical abstract: Indigo production identifies hotspots in cytochrome P450 BM3 for diversifying aromatic hydroxylation
From the themed collection: Biocatalysis
Open Access Paper

Concluding remarks: biocatalysis

The classes of compounds accessible via biocatalysis are as diverse as the concepts to make them, as presented at the Faraday Discussion on biocatalysis.

Graphical abstract: Concluding remarks: biocatalysis
From the themed collection: Biocatalysis
Open Access Paper

Retuning the potential of the electrochemical leaf

By changing an active site tyrosine in FNR (the enzyme central to the e-Leaf) its exclusivity for NADP(H) swaps to NAD(H). Here, an electrochemical study of this variant reveals that the reduction potential of its active site flavin has been retuned.

Graphical abstract: Retuning the potential of the electrochemical leaf
From the themed collection: Biocatalysis
Open Access Paper

Surveying the scope of aromatic decarboxylations catalyzed by prenylated-flavin dependent enzymes

H/D exchange between substrate and solvent D2O provides a sensitive test for substrate activation in prenylated-flavin dependent enzymes. We show that a remarkably diverse range of molecules can be activated by one enzyme, ferulic acid decarboxylase.

Graphical abstract: Surveying the scope of aromatic decarboxylations catalyzed by prenylated-flavin dependent enzymes
From the themed collection: Biocatalysis
Open Access Paper

High-throughput selection of (new) enzymes: phage display-mediated isolation of alkyl halide hydrolases from a library of active-site mutated epoxide hydrolases

Epoxide hydrolase StEH1 was subjected to targeted randomized mutagenesis at five active-site amino acid residues and the resulting protein library was challenged for reactivity towards a bait chloride substrate.

Graphical abstract: High-throughput selection of (new) enzymes: phage display-mediated isolation of alkyl halide hydrolases from a library of active-site mutated epoxide hydrolases
From the themed collection: Biocatalysis
Open Access Paper

Investigating the effect of fusion partners on the enzymatic activity and thermodynamic stability of poly(ethylene terephthalate) degrading enzymes

We investigate the influence of the switchable thermal stability of a fusion partner on the activity of PETases demonstrating a range of optimal reaction temperatures.

Graphical abstract: Investigating the effect of fusion partners on the enzymatic activity and thermodynamic stability of poly(ethylene terephthalate) degrading enzymes
From the themed collection: Biocatalysis
Open Access Paper

An engineered T7 RNA polymerase for efficient co-transcriptional capping with reduced dsRNA byproducts in mRNA synthesis

We have engineered a novel RNA polymerase, T7-68, that improves co-transcriptional incorporation of selected cap analogs and reduces dsRNA content, addressing manufacturing challenges and facilitating large-scale mRNA production.

Graphical abstract: An engineered T7 RNA polymerase for efficient co-transcriptional capping with reduced dsRNA byproducts in mRNA synthesis
From the themed collection: Biocatalysis
Open Access Paper

On synergy between ultrahigh throughput screening and machine learning in biocatalyst engineering

We explore strategies for drawing-up ‘fitness landscapes’ in sequence space with ultrahigh throughput (uHT) droplet microfluidics, review the current state of AI/ML in enzyme engineering and discuss how uHT datasets may be combined with AI/ML.

Graphical abstract: On synergy between ultrahigh throughput screening and machine learning in biocatalyst engineering
From the themed collection: Biocatalysis
Open Access Paper

Computation-guided engineering of distal mutations in an artificial enzyme

In silico prediction of distal hotspots was used to introduce distal mutations in an artificial enzyme that improved its reactivity and thermostability by shifting its conformational distribution.

Graphical abstract: Computation-guided engineering of distal mutations in an artificial enzyme
From the themed collection: Biocatalysis
Paper

Enhancement of essential cofactors for in vivo biocatalysis

Boosting the biosynthesis of essential cofactors by addition of xylose reductase and lactose to enhance product synthesis using synthetic biology.

Graphical abstract: Enhancement of essential cofactors for in vivo biocatalysis
From the themed collection: Biocatalysis
Open Access Paper

Harnessing conformational dynamics in enzyme catalysis to achieve nature-like catalytic efficiencies: the shortest path map tool for computational enzyme redesign

It is challenging to identify enzyme mutations that enhance specific conformational changes.We develop the shortest path map method to address this challenge.

Graphical abstract: Harnessing conformational dynamics in enzyme catalysis to achieve nature-like catalytic efficiencies: the shortest path map tool for computational enzyme redesign
From the themed collection: Biocatalysis
Open Access Paper

Computational study of the mechanism of a polyurethane esterase A (PueA) from Pseudomonas chlororaphis

We investigate the possible molecular mechanism of polyurethane esterase A, previously identified as responsible for degradation of a polyester polyurethane sample in Pseudomonas chlororaphis.

Graphical abstract: Computational study of the mechanism of a polyurethane esterase A (PueA) from Pseudomonas chlororaphis
From the themed collection: Biocatalysis
Open Access Paper

Designing Michaelases: exploration of novel protein scaffolds for iminium biocatalysis

The study identified promising protein scaffolds for artificial enzyme development in iminium-ion catalysis, demonstrating activity and enantioselectivity in abiological Michael addition reactions.

Graphical abstract: Designing Michaelases: exploration of novel protein scaffolds for iminium biocatalysis
From the themed collection: Biocatalysis
Open Access Paper

An efficient pyrrolysyl-tRNA synthetase for economical production of MeHis-containing enzymes

A highly efficient aminoacyl tRNA synthetase (G1PylRSMIFAF) has been developed to produce MeHis-containing proteins. High protein titres can be achieved with low ncAA concentrations (0.1 mM) enabling more economical production of MeHis-containing enzymes.

Graphical abstract: An efficient pyrrolysyl-tRNA synthetase for economical production of MeHis-containing enzymes
From the themed collection: Biocatalysis
Paper

Tuning the peroxidase activity of artificial P450 peroxygenase by engineering redox-sensitive residues

Engineering redox-sensitive residues dramatically increases the peroxidase activity of P450BM3 monooxygenase, providing new insights and a strategy for regulating P450s' promiscuous functionality.

Graphical abstract: Tuning the peroxidase activity of artificial P450 peroxygenase by engineering redox-sensitive residues
From the themed collection: Biocatalysis
Open Access Paper

Degradation of PET microplastic particles to monomers in human serum by PETase

This study provides the first evidence of hydrolysis of PET microplastics in human serum by esterases, to combat the microplastic contamination in humans.

Graphical abstract: Degradation of PET microplastic particles to monomers in human serum by PETase
From the themed collection: Biocatalysis
Open Access Paper

Developing deprotectase biocatalysts for synthesis

A 1 pot, 2 step deprotection biocatalytic cascade.

Graphical abstract: Developing deprotectase biocatalysts for synthesis
From the themed collection: Biocatalysis
Open Access Paper

Friends and relatives: insight into conformational regulation from orthologues and evolutionary lineages using KIF and KIN

We have developed novel tools to characterize evolutionarily conserved non-covalent interactions in proteins. We showcase their application to understanding substrate specificity in class A β-lactamases, with potential impact for protein engineering.

Graphical abstract: Friends and relatives: insight into conformational regulation from orthologues and evolutionary lineages using KIF and KIN
From the themed collection: Biocatalysis
Open Access Paper

Exploring the selectivity of cytochrome P450 for enhanced novel anticancer agent synthesis

This work exemplifies the use of machine-learned 3D structure predictors combined with molecular docking to generate poses for atomistic study via molecular dynamics, ultimately enabling rapid insight into a novel biosynthetic cascade.

Graphical abstract: Exploring the selectivity of cytochrome P450 for enhanced novel anticancer agent synthesis
From the themed collection: Biocatalysis
Open Access Paper

Oxygen-resistant [FeFe]hydrogenases: new biocatalysis tools for clean energy and cascade reactions

A game-changer [FeFe]hydrogenase was studied to dissect the mechanism of oxygen resistance and learn how to protect biocatalysts for hydrogen-driven reactions.

Graphical abstract: Oxygen-resistant [FeFe]hydrogenases: new biocatalysis tools for clean energy and cascade reactions
From the themed collection: Biocatalysis
Open Access Paper

Towards controlling activity of a peptide asparaginyl ligase (PAL) by lumazine synthetase compartmentalization

We aim to compartmentalize a representative peptide asparaginyl ligase, OaAEP1-C247A, within protein containers to create artificial organelles with substrate sorting capability.

Graphical abstract: Towards controlling activity of a peptide asparaginyl ligase (PAL) by lumazine synthetase compartmentalization
From the themed collection: Biocatalysis
Open Access Paper

On the biocatalytic synthesis of silicone polymers

A biocatalytic approach for polydimethylsiloxane synthesis is demonstrated using silicatein-α, an enzyme from marine sponges that catalyses the hydrolysis and condensation of Si–O bonds.

Graphical abstract: On the biocatalytic synthesis of silicone polymers
From the themed collection: Biocatalysis
Paper

Application of rational enzyme engineering in a new route to etonogestrel and levonorgestrel: carbonyl reductase bioreduction of ethyl secodione

We combine the selection and engineering of a carbonyl reductase enzyme with process development, to enable efficient and economically viable bioreduction of ethyl secodione to (13R,17S)-secol, the key chirality introducing intermediate en route to contraceptives etonogestrel and levonorgestrel.

Graphical abstract: Application of rational enzyme engineering in a new route to etonogestrel and levonorgestrel: carbonyl reductase bioreduction of ethyl secodione
From the themed collection: Biocatalysis
Discussion

Biocatalysis for industry, medicine and the circular economy: general discussion

From the themed collection: Biocatalysis
Discussion

Enzyme evolution, engineering and design: mechanism and dynamics: general discussion

From the themed collection: Biocatalysis
Discussion

Artificial, biomimetic and hybrid enzymes: general discussion

From the themed collection: Biocatalysis
Discussion

Biocatalytic pathways, cascades, cells and systems: general discussion

From the themed collection: Biocatalysis
30 items

About this collection

We are delighted to share with you a selection of the papers associated with a Faraday Discussion on Biocatalysis. More information about the related event may be found here: http://rsc.li/biocatalysis-fd2024. Additional articles will be added to the collection as they are published. The final versions of all the articles presented and a record of the discussions will be published after the event.

Biocatalysis, a rapidly evolving field with increasing impact in synthesis, chemical manufacturing and medicine, is being transformed by advances in biophysical and computational techniques. It is expanding into new areas of chemistry, facilitated by de novo protein design, directed evolution, incorporation of new catalytic functionality into proteins, genetic data, developing spectroscopic and structural techniques, and informed by modelling, machine learning and artificial intelligence. The four themes of this Discussion will unite researchers working in different fields (for example synthetic biology, computational chemistry and mechanistic organic and organometallic chemistry) to improve our understanding of enzyme catalytic power with a view to engineering hybrid and artificial enzymes.

This meeting will cover 4 main themes: Enzyme evolution, engineering and design: mechanism and dynamics, Biocatalytic pathways, cascades, cells and systems, Biocatalysis for industry, medicine and the circular economy, Artificial, biomimetic and hybrid enzymes.

On behalf of the Scientific Committee, we hope you join us and participate in this exciting event, and that you enjoy these articles and the record of the discussion.

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