Many of today’s scientific questions are complex and therefore most fruitfully studied by multidisciplinary approaches that extend beyond the traditional boundaries of research. As cartilage is the load-bearing tissue in a complex organ (the joint) that offers mechanical functions to the body, biomechanical properties (e.g. compressive modulus) of the tissue should be the ultimate measures of the tissue’s health. However, the biomechanical properties of cartilage are determined at the molecular level by the amounts and spatial arrangements of biomolecules as well as the interactions between them. Furthermore, the environment in which these biomolecules exist is regulated at the cellular level by the cartilage cells, chondrocytes. In cartilage, these cells are spatially confined at the morphological level by the architectures of the extracellular matrices. These multiscale features of cartilage certainly suggest the need for a wide range of research tools and approaches. While nuclear magnetic resonance (NMR) and magnetic resonance imaging (MRI) are firmly established as tools to assess cartilage integrity and strength, several imaging techniques that could provide complementary information to NMR and MRI in cartilage research are discussed in this chapter. These techniques include polarized light microscopy, Fourier-transform infrared imaging, transmission electron microscopy, microscopic computed tomography and several additional imaging techniques. A final graphical summary illustrates some of the benefits and correlations derived from this type of multi-modality approach.