Issue 23, 2021

MicroRNA-5106-based nanodelivery to enhance osteogenic differentiation and bone regeneration of bone mesenchymal stem cells through targeting of Gsk-3α

Abstract

Regulating bone mesenchymal stem cell (BMSC) differentiation into osteoblastic lineage plays a significant role in bone tissue regeneration. MicroRNAs are highly conserved small noncoding RNAs that mediate some special cell behaviors through repressing or upregulating the mRNA expression of their target genes. Here, we show that microRNA-5106 (miR-5106) modulates and enhances the osteoblastic differentiation of BMSCs through liposome-based delivery and transfection. The results revealed that miR-5106 was up-regulated during the differentiation of BMSCs. The overexpression of miR-5106 significantly promoted the alkaline phosphatase (ALP) activity, expressions of osteoblast-specific genes and extracellular matrix mineralization, whereas the inhibition of miR-5106 functioned by anti-miR-5106 (miR-5106 inhibitor) significantly decreased the osteoblastic differentiation of BMSCs. Further studies indicated that miR-5106 enhances the osteogenic differentiation of BMSCs through inhibiting Gsk-3α in the Wnt/β-catenin pathway. The delivery of the miR-5106 complex significantly enhanced the critical-sized bone defect healing in vivo. We anticipate that our findings could provide a new miRNA-based therapeutic method and an osteoblastic molecular mechanism for enhancing bone tissue regeneration.

Graphical abstract: MicroRNA-5106-based nanodelivery to enhance osteogenic differentiation and bone regeneration of bone mesenchymal stem cells through targeting of Gsk-3α

Supplementary files

Article information

Article type
Research Article
Submitted
06 мар 2021
Accepted
16 июн 2021
First published
26 окт 2021

Mater. Chem. Front., 2021,5, 8138-8150

MicroRNA-5106-based nanodelivery to enhance osteogenic differentiation and bone regeneration of bone mesenchymal stem cells through targeting of Gsk-3α

M. Yu and B. Lei, Mater. Chem. Front., 2021, 5, 8138 DOI: 10.1039/D1QM00367D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements