Issue 6, 2019

Laccase from Botryosphaeria rhodina MAMB-05 as a biological component in electrochemical biosensing devices

Abstract

In this work a crude laccase preparation from Botryosphaeria rhodina MAMB-05 was employed to construct an electrochemical biosensor using nanostructured carbon black. The biosensor was evaluated by differential pulse voltammetry under optimized conditions and was utilized in the determination of epinephrine (EPN). The biosensor’s storage stability was evaluated and was found to be stable over seven consecutive days with a reduction of only 7.0% in the EPN response. Under the optimum conditions developed, the biosensor provided a linear current response for the concentration of EPN in the range from 4.98 to 285 μM. The biosensor was successfully applied for determining EPN in pharmaceutical-grade EPN injection samples, and in synthetic cerebrospinal fluid, demonstrating its promising analytical performance in drug analysis.

Graphical abstract: Laccase from Botryosphaeria rhodina MAMB-05 as a biological component in electrochemical biosensing devices

Article information

Article type
Communication
Submitted
24 дек 2018
Accepted
05 янв 2019
First published
07 янв 2019

Anal. Methods, 2019,11, 717-720

Laccase from Botryosphaeria rhodina MAMB-05 as a biological component in electrochemical biosensing devices

J. T. Moraes, C. A. R. Salamanca-Neto, Ĺubomír Švorc, J. G. Schirmann, A. M. Barbosa-Dekker, R. F. H. Dekker and E. R. Sartori, Anal. Methods, 2019, 11, 717 DOI: 10.1039/C8AY02805B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements