Issue 3, 2019

New mass spectrometry technologies contributing towards comprehensive and high throughput omics analyses of single cells

Abstract

Mass-spectrometry based omics technologies – namely proteomics, metabolomics and lipidomics – have enabled the molecular level systems biology investigation of organisms in unprecedented detail. There has been increasing interest for gaining a thorough, functional understanding of the biological consequences associated with cellular heterogeneity in a wide variety of research areas such as developmental biology, precision medicine, cancer research and microbiome science. Recent advances in mass spectrometry (MS) instrumentation and sample handling strategies are quickly making comprehensive omics analyses of single cells feasible, but key breakthroughs are still required to push through remaining bottlenecks. In this review, we discuss the challenges faced by single cell MS-based omics analyses and highlight recent technological advances that collectively can contribute to comprehensive and high throughput omics analyses in single cells. We provide a vision of the potential of integrating pioneering technologies such as Structures for Lossless Ion Manipulations (SLIM) for improved sensitivity and resolution, novel peptide identification tactics and standards free metabolomics approaches for future applications in single cell analysis.

Graphical abstract: New mass spectrometry technologies contributing towards comprehensive and high throughput omics analyses of single cells

Article information

Article type
Minireview
Submitted
15 авг 2018
Accepted
14 ноя 2018
First published
03 дек 2018

Analyst, 2019,144, 794-807

Author version available

New mass spectrometry technologies contributing towards comprehensive and high throughput omics analyses of single cells

S. P. Couvillion, Y. Zhu, G. Nagy, J. N. Adkins, C. Ansong, R. S. Renslow, P. D. Piehowski, Y. M. Ibrahim, R. T. Kelly and T. O. Metz, Analyst, 2019, 144, 794 DOI: 10.1039/C8AN01574K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements