SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range

Y. G. Ermakova; V. V. Pak; Y. A. Bogdanova; A. A. Kotlobay; I. V. Yampolsky; A. G. Shokhina; A. S. Panova; R. A. Marygin; D. B. Staroverov; D. S. Bilan; H. Sies; V. V. Belousov

doi:10.1039/C7CC08740C

SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range†

Y. G. Ermakova,^ab V. V. Pak,^a Y. A. Bogdanova,^a A. A. Kotlobay,^a I. V. Yampolsky,^a A. G. Shokhina,^a A. S. Panova,^ac R. A. Marygin,

^a D. B. Staroverov,^a D. S. Bilan,^ab H. Sies^de and V. V. Belousov

*^abf

Author affiliations

* Corresponding authors

^a Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia
E-mail: belousov@ibch.ru

^b Pirogov Russian National Research Medical University, Moscow, Russia

^c Department of Biochemistry, Biological Faculty, Lomonosov Moscow State University, Moscow, Russia

^d Institute of Biochemistry and Molecular Biology I, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany

^e Leibniz Institute for Environmental Medicine, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany

^f Institute for Cardiovascular Physiology, Georg-August-University Göttingen, Göttingen, Germany

Abstract

We designed a genetically encoded ratiometric fluorescent probe, SypHer3s, with enhanced brightness and optimized pK_a, which responds to pH changes in different cellular compartments. SypHer3s was successfully utilized for imaging the pH dynamics in mitochondria of living neurons and in quantitative pH measurement in zebrafish embryos.

This article is part of the themed collection: Biological optical imaging

Chemical Communications

SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range†

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