Issue 20, 2017

Stereolithographic printing of ionically-crosslinked alginate hydrogels for degradable biomaterials and microfluidics

Abstract

3D printed biomaterials with spatial and temporal functionality could enable interfacial manipulation of fluid flows and motile cells. However, such dynamic biomaterials are challenging to implement since they must be responsive to multiple, biocompatible stimuli. Here, we show stereolithographic printing of hydrogels using noncovalent (ionic) crosslinking, which enables reversible patterning with controlled degradation. We demonstrate this approach using sodium alginate, photoacid generators and various combinations of divalent cation salts, which can be used to tune the hydrogel degradation kinetics, pattern fidelity, and mechanical properties. This approach is first utilized to template perfusable microfluidic channels within a second encapsulating hydrogel for T-junction and gradient devices. The presence and degradation of printed alginate microstructures were further verified to have minimal toxicity on epithelial cells. Degradable alginate barriers were used to direct collective cell migration from different initial geometries, revealing differences in front speed and leader cell formation. Overall, this demonstration of light-based 3D printing using non-covalent crosslinking may enable adaptive and stimuli-responsive biomaterials, which could be utilized for bio-inspired sensing, actuation, drug delivery, and tissue engineering.

Graphical abstract: Stereolithographic printing of ionically-crosslinked alginate hydrogels for degradable biomaterials and microfluidics

Supplementary files

Article information

Article type
Paper
Submitted
03 июл 2017
Accepted
05 сен 2017
First published
05 сен 2017

Lab Chip, 2017,17, 3474-3488

Stereolithographic printing of ionically-crosslinked alginate hydrogels for degradable biomaterials and microfluidics

T. M. Valentin, S. E. Leggett, P. Chen, J. K. Sodhi, L. H. Stephens, H. D. McClintock, J. Y. Sim and I. Y. Wong, Lab Chip, 2017, 17, 3474 DOI: 10.1039/C7LC00694B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements