Issue 24, 2018

Acoustic impedance-based size-independent isolation of circulating tumour cells from blood using acoustophoresis

Abstract

Label-free isolation of CTCs from blood is critical for the development of diagnostic and prognostic tools for cancer. Here, we report a label-free method based on acoustic impedance contrast for the isolation of CTCs from peripheral blood mononuclear cells (PBMCs) in a microchannel using acoustophoresis. We describe a method in which the acoustophoretic migration of PBMCs is arrested by matching their acoustic impedance with that of the sample medium, and CTCs that have different acoustic impedance compared to PBMCs migrate toward the pressure node or antinode and thus become isolated. We show that acoustic streaming which can adversely affect the CTC isolation is suppressed owing to the inhomogeneous liquid flow configuration. We establish a method for isolation of CTCs that have higher or lower acoustic impedance compared to PBMCs by controlling the acoustic impedance contrast of the liquids across the channel. Applying this method, we demonstrate label-free isolation of HeLa and MDA-MB-231 cells from PBMCs (collected from 2.0 mL of blood) within one hour yielding a recovery of >86% and >50-fold enrichment. Combined impedance and size-based sorting is proposed as a promising tool for the effective isolation of CTCs from blood.

Graphical abstract: Acoustic impedance-based size-independent isolation of circulating tumour cells from blood using acoustophoresis

Supplementary files

Article information

Article type
Paper
Submitted
30 авг 2018
Accepted
29 окт 2018
First published
30 окт 2018

Lab Chip, 2018,18, 3802-3813

Acoustic impedance-based size-independent isolation of circulating tumour cells from blood using acoustophoresis

S. Karthick, P. N. Pradeep, P. Kanchana and A. K. Sen, Lab Chip, 2018, 18, 3802 DOI: 10.1039/C8LC00921J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements