Issue 42, 2017

Substoichiometric ribose methylations in spliceosomal snRNAs

Abstract

Sequencing-based profiling of ribose methylations is a new approach that allows for experiments addressing dynamic changes on a large scale. Here, we apply such a method to spliceosomal snRNAs present in human whole cell RNA. Analysis of solid tissue samples confirmed all previously known sites and demonstrated close to full methylation at almost all sites. Methylation changes were revealed in biological experimental settings, using T cell activation as an example, and in the T cell leukemia model, Jurkat cells. Such changes could impact the dynamics of snRNA interactions during the spliceosome cycle and affect mRNA splicing efficiency and splicing patterns.

Graphical abstract: Substoichiometric ribose methylations in spliceosomal snRNAs

Supplementary files

Article information

Article type
Communication
Submitted
16 сен 2017
Accepted
13 окт 2017
First published
13 окт 2017

Org. Biomol. Chem., 2017,15, 8872-8876

Substoichiometric ribose methylations in spliceosomal snRNAs

N. Krogh, M. Kongsbak-Wismann, C. Geisler and H. Nielsen, Org. Biomol. Chem., 2017, 15, 8872 DOI: 10.1039/C7OB02317K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements