Issue 10, 2016

Characterization and simultaneous quantification of seven triterpenoid saponins in different parts of Xanthoceras sorbifolia Bunge by HPLC-ESI-TOF

Abstract

In order to find new candidates against Alzheimer's disease, two new triterpenoid saponins, sorbifoside C (1) and D (4), and five known triterpenoid saponins (2, 3, and 5–7) were isolated and identified from the husks of Xanthoceras sorbifolia Bunge. Their structures were elucidated through 1D, 2D NMR and HR-MS. A fast, sensitive and reliable high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS) method has been developed and validated to simultaneously quantitate the content of seven triterpenoid saponins in different parts of X. sorbifolia. The quantification was accomplished on a triple quadrupole tandem mass spectrometer in the selected ion monitoring and positive ionization modes. Good chromatographic separation was achieved by gradient elution with the mobile phase consisting of acetonitrile and 0.05% formic acid in water during a total run time of 20 min. All of the seven compounds showed good linearity (r2 > 0.998) in relatively wide concentration ranges. Satisfactory precisions (evaluated by intra- and inter-day tests) and recoveries/accuracies (98.0% to 102.0%) were obtained with RSD values less than 3.0%. The method was successfully adapted for simultaneous quantification of the seven analytes in different parts of X. sorbifolia. The results showed that triterpenoid saponin contents from different parts of the plant varied significantly and a reference for the choice of medicinal parts was accordingly provided.

Graphical abstract: Characterization and simultaneous quantification of seven triterpenoid saponins in different parts of Xanthoceras sorbifolia Bunge by HPLC-ESI-TOF

Article information

Article type
Paper
Submitted
25 окт 2015
Accepted
03 фев 2016
First published
04 фев 2016

Anal. Methods, 2016,8, 2176-2184

Characterization and simultaneous quantification of seven triterpenoid saponins in different parts of Xanthoceras sorbifolia Bunge by HPLC-ESI-TOF

W. Rong, Z. Sun, Q. Li, R. Liu, T. Zhang, T. Wang, W. Yang, Z. Li and K. Bi, Anal. Methods, 2016, 8, 2176 DOI: 10.1039/C5AY02813B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements