Assessing the impact of food-derived bioactives on digestive proteases by in vitro and in silico approaches

Sara Margherita Borgonovi, Florinda Perugino, Luca Dellafiora, Francesca Annunziata, Lorenzo Pedroni, Gianni Galaverna, Andrea Pinto, Sabrina Dallavalle, Stefania Iametti and Mattia Di Nunzio

Food Funct., 2025, 16, 2959
DOI: 10.1039/D4FO04022H

Pea protein extraction method impacts the protein (micro)structural organisation and in vitro digestion kinetics

Sarah H. E. Verkempinck, Dorine Duijsens, Ankita Mukherjee and Peter J. Wilde

Food Funct., 2024, 15, 953
DOI: 10.1039/D3FO04225A

In vitrodigestion of protein and starch in sponge cakes formulated with pea (Pisum sativumL.) ingredients

Svenja Krause, Stéphane Debon, Katharina Pälchen, Ralf Jakobi, Barbara Rega, Catherine Bonazzi and Tara Grauwet

Food Funct., 2022, 13, 3206
DOI: 10.1039/D1FO03601G

Targeted hydrothermally induced cell biopolymer changes explain the in vitro digestion of starch and proteins in common bean (Phaseolus vulgaris) cotyledons

Erick O. Okelo, Irene Wainaina, Dorine Duijsens, Arnold Onyango, Daniel Sila, Tara Grauwet and Marc E. G. Hendrickx

Food Funct., 2024, 15, 8848
DOI: 10.1039/D4FO00734D

Novel nano-encapsulated probiotic agents: Encapsulate materials, delivery, and encapsulation systems

Cong Xu, Qingfeng Ban, Wan Wang, Juncai Hou and Zhanmei Jiang

Journal of Controlled Release, 2022, 349, 184
DOI: 10.1016/j.jconrel.2022.06.061

Computational characteristics of kinetic models for in vitro protein digestion: A review

Peter Adeoye Sopade

Journal of Food Engineering, 2024, 360, 111690
DOI: 10.1016/j.jfoodeng.2023.111690

An Updated Review on the in vitro and Computational Approaches for the Simulation of Carbohydrate Digestion

Tong Wang, Shijun Zhu, Ji Lang, Nan Shang and Wang Liao

Food Reviews International, 2025, 41, 2167
DOI: 10.1080/87559129.2025.2509292

Assessing the digestibility and estimated bioavailability/ bioaccessibility of plant-based proteins and minerals from soy, pea, and faba bean ingredients

Jaqueline Auer, Marie Alminger, Marina Marinea, Mathias Johansson, Galia Zamaratskaia, Anders Högberg and Maud Langton

LWT, 2024, 197, 115893
DOI: 10.1016/j.lwt.2024.115893

Cell wall permeability in relation to in vitro starch digestion of pea cotyledon cells

Abayomi Ajala, Lovedeep Kaur, Sung Je Lee, Patrick J.B. Edwards and Jaspreet Singh

Food Structure, 2024, 41, 100381
DOI: 10.1016/j.foostr.2024.100381

Understanding the impact of different post-digestion enzymatic inhibition methods and storage conditions on standardized gastrointestinal model (INFOGEST): A case study of Pleurotus ostreatus mushroom biomass

Helena Araújo-Rodrigues, Ana Sofia Salsinha, Ezequiel R. Coscueta, João Bettencourt Relvas, Freni K. Tavaria and Manuela E. Pintado

Food Research International, 2025, 211, 116432
DOI: 10.1016/j.foodres.2025.116432

Protein accessibility level affects macronutrient digestion kinetics of plant-based shakes

J.M. Guevara-Zambrano, S.H.E. Verkempinck, B. Muriithi, D. Duijsens, M.E. Hendrickx, A.M. Van Loey and T. Grauwet

Food Hydrocolloids, 2023, 137, 108428
DOI: 10.1016/j.foodhyd.2022.108428

In vitro protein bioaccessibility and human serum amino acid responses to white bread enriched with intact plant cells

Natalia Perez-Moral, Shikha Saha, Ana M. Pinto, Balazs H. Bajka and Cathrina H. Edwards

Food Chemistry, 2023, 404, 134538
DOI: 10.1016/j.foodchem.2022.134538

Impact of the stepwise implementation of INFOGEST semi-dynamic conditions on in vitro starch and protein digestion: A case study on lentil cotyledon cells

D. Duijsens, S.H.E. Verkempinck and T. Grauwet

Food Research International, 2024, 197, 115214
DOI: 10.1016/j.foodres.2024.115214

Quantifying protein digestion kinetics of feed ingredients using a modified in vitro incubation assay

Shiyi Zhang, Leon de Jonge, Sonja de Vries and Walter J J Gerrits

Journal of Animal Science, 2025, 103
DOI: 10.1093/jas/skaf190

Cell wall of feeds and their impact on protein digestibility: An in vitro method applied for pig nutrition

Myriam M.L. Grundy, Julie Tang, Jaap van Milgen and David Renaudeau

Animal Feed Science and Technology, 2022, 293, 115467
DOI: 10.1016/j.anifeedsci.2022.115467

Digestion kinetics of lipids and proteins in plant-based shakes: Impact of processing conditions and resulting structural properties

J.M. Guevara-Zambrano, S.H.E. Verkempinck, L. Hernandez-Ruiz, M.R. Infantes-Garcia, M.E. Hendrickx, A.M. Van Loey and T. Grauwet

Food Chemistry, 2022, 382, 132306
DOI: 10.1016/j.foodchem.2022.132306

Effect of cinnamon on starch hydrolysis of rice pudding: Comparing static and dynamic in vitro digestion models

Yiwen Li, Rui Xu, Haning Xiu, Jiannan Feng, Hea Jin Park, Himanshu Prabhakar and Fanbin Kong

Food Research International, 2022, 161, 111813
DOI: 10.1016/j.foodres.2022.111813

Gastric and small intestinal lipid digestion kinetics as affected by the gradual addition of lipases and bile salts

S.H.E. Verkempinck, J.M. Guevara-Zambrano, M.R. Infantes-Garcia, M.C. Naranjo, R. Soliva-Fortuny, P. Elez-Martínez and T. Grauwet

Food Bioscience, 2022, 46, 101595
DOI: 10.1016/j.fbio.2022.101595

Engineering strategies to modulate nutrient digestion kinetics and bioaccessibility of plant-based foods

Sarah HE Verkempinck, Marc EG Hendrickx, Ann Van Loey and Tara Grauwet

Current Opinion in Food Science, 2023, 52, 101052
DOI: 10.1016/j.cofs.2023.101052

Solubility, (micro)structure, and in vitro digestion of pea protein dispersions as affected by high pressure homogenization and environmental conditions

J.M. Guevara-Zambrano, P. Chowdhury, A.G.B. Wouters and S.H.E. Verkempinck

Food Research International, 2024, 188, 114434
DOI: 10.1016/j.foodres.2024.114434

Influence of flour extraction rate on static in vitro gastrointestinal digestion characteristics of wheat protein during conventional milling

Lingfang Zhang, Shuzhen Yang, Binghua Sun, Xiaoxi Wang and Sen Ma

International Journal of Food Science and Technology, 2024, 59, 4608
DOI: 10.1111/ijfs.17184

Effect of experimental flour preparation and thermal treatment on the volatile properties of aqueous chickpea flour suspensions

Laura E.C. Noordraven, Carolien Buvé, Tara Grauwet and Ann M. Van Loey

LWT, 2022, 160, 113171
DOI: 10.1016/j.lwt.2022.113171

Importance of adapted digestion conditions to simulate in vitro lipid digestion of broilers in different life stages

Daphne Michels, Sarah H.E. Verkempinck, Agnese Panozzo, Karen Vermeulen, Marc E. Hendrickx, Liesbet Thijs and Tara Grauwet

Animal Nutrition, 2023, 12, 151
DOI: 10.1016/j.aninu.2022.09.008

In vitro gastric digestion of polysaccharides in mixed dispersions: Evaluating the contribution of human salivary α-amylase on starch molecular breakdown

M. Torp Nielsen, L. Roman and M. Corredig

Current Research in Food Science, 2024, 8, 100759
DOI: 10.1016/j.crfs.2024.100759

Moderately mechanically activated starch in improving protein digestibility: Application in noodles

Lingfang Zhang, Shuzhen Yang, Congcong Wang, Qianyi Jiang, Xiaoxi Wang and Binghua Sun

International Journal of Biological Macromolecules, 2025, 298, 139856
DOI: 10.1016/j.ijbiomac.2025.139856

Monitoring furanic and dicarbonyl compounds in pea-based and wheat-based sponge cakes during in vitro digestion

Federica Secco, Even Le Roux, Véronique Bosc and Barbara Rega

Food Research International, 2025, 208, 116099
DOI: 10.1016/j.foodres.2025.116099

Utilizing Hydrothermal Processing to Align Structure and In Vitro Digestion Kinetics between Three Different Pulse Types

Katharina Pälchen, Ben Van den Wouwer, Dorine Duijsens, Marc E. Hendrickx, Ann Van Loey and Tara Grauwet

Foods, 2022, 11, 206
DOI: 10.3390/foods11020206

A basis for IgY-themed functional foods: Digestion profile of oral yolk immunoglobulin (IgY) by INFOGEST static digestion model

Minquan Xia, Dong Uk Ahn, Cong Liu and Zhaoxia Cai

Food Research International, 2022, 162, 112167
DOI: 10.1016/j.foodres.2022.112167

Effect of processing and microstructural properties of chickpea-flours on in vitro digestion and appetite sensations

Katharina Pälchen, Wender L.P. Bredie, Dorine Duijsens, Alan Isaac Alfie Castillo, Marc Hendrickx, Ann Van Loey, Anne Raben and Tara Grauwet

Food Research International, 2022, 157, 111245
DOI: 10.1016/j.foodres.2022.111245

Unravelling the impact of emulsifier blends on interfacial properties and in vitro small intestinal lipolysis of oil-in-water emulsions

Daphne Michels, Sarah H.E. Verkempinck, Esther Staes, Riet Spaepen, Karen Vermeulen, Alexandra Wealleans and Tara Grauwet

Food Hydrocolloids, 2023, 141, 108735
DOI: 10.1016/j.foodhyd.2023.108735

Native and processed legume seed microstructure and its influence on starch digestion and glycaemic features: A review

Abayomi Ajala, Lovedeep Kaur, Sung Je Lee and Jaspreet Singh

Trends in Food Science & Technology, 2023, 133, 65
DOI: 10.1016/j.tifs.2023.01.011

Enhanced secretion of satiety-promoting gut hormones in healthy humans after consumption of white bread enriched with cellular chickpea flour: A randomized crossover study

Balazs H. Bajka, Ana M. Pinto, Natalia Perez-Moral, Shikha Saha, Peter Ryden, Jennifer Ahn-Jarvis, Alice van der Schoot, Catherine Bland, Sarah E. Berry, Peter R. Ellis and Cathrina H. Edwards

The American Journal of Clinical Nutrition, 2023, 117, 477
DOI: 10.1016/j.ajcnut.2022.12.008

Starch digestion: A comprehensive update on the underlying modulation mechanisms and its in vitro assessment methodologies

Daniela Freitas, Athina Lazaridou, Dorine Duijsens, Kali Kotsiou, Kendall R. Corbin, Marilisa Alongi, Natalia Perez-Moral, Sebnem Simsek, Sedef Nehir El, Shannon Gwala, Sibel Karakaya, Steven Le Feunteun, Tara Grauwet, Mario M. Martinez and Cathrina H. Edwards

Trends in Food Science & Technology, 2025, 159, 104969
DOI: 10.1016/j.tifs.2025.104969

Co-ingestion of Cereals and Legumes during Infant Complementary Feeding: Starch and Protein in vitro Digestion

Marianela D. Rodriguez, Alberto E. León and Mariela C. Bustos

Plant Foods Hum Nutr, 2024, 79, 489
DOI: 10.1007/s11130-024-01170-1