Issue 6, 2019

Seven-enzyme in vitro cascade to (3R)-3-hydroxybutyryl-CoA

Abstract

Economical and environmentally-friendly routes to convert feedstock chemicals like acetate into valuable chiral products such as (R)-3-hydroxybutyrate are in demand. Here, seven enzymes (CoaA, CoaD, CoaE, ACS, BktB, PhaB, and GDH) are employed in a one-pot, in vitro, biocatalytic synthesis of (3R)-3-hydroxybutyryl-CoA, which was readily isolated. This platform generates not only chiral diketide building blocks but also desirable CoA derivatives.

Graphical abstract: Seven-enzyme in vitro cascade to (3R)-3-hydroxybutyryl-CoA

Supplementary files

Article information

Article type
Communication
Submitted
15 nov 2018
Accepted
09 jan 2019
First published
09 jan 2019

Org. Biomol. Chem., 2019,17, 1375-1378

Seven-enzyme in vitro cascade to (3R)-3-hydroxybutyryl-CoA

L. E. Valencia, Z. Zhang, A. J. Cepeda and A. T. Keatinge-Clay, Org. Biomol. Chem., 2019, 17, 1375 DOI: 10.1039/C8OB02858C

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