Issue 11, 2023

Exosomes trapping, manipulation and size-based separation using opto-thermo-electrohydrodynamic tweezers

Abstract

Owing to the heterogeneity of exosomes in size and biomolecular composition, there is a need for new approaches for trapping, manipulating, and sorting of single exosomes in solution. Due to their small size ranging from 30 nm to 150 nm and their relatively low refractive index, their stable trapping using optical tweezers has been met with challenges. Trapping exosomes in an optical trap requires nearly 100 mW of input power, which predisposes them to photo-induced damage and membrane rupture at the laser focus. Here, we report a high stability opto-thermo-electrohydrodynamic tweezer for the stable stand-off trapping of single exosomes based on a concentric nanohole array (CNA) using laser illumination and an a.c. field. The CNA system generates two regions of electrohydrodynamic potentials several microns away from the laser focus where single exosomes are trapped. We demonstrate the rapid trapping within seconds, and selective dynamic manipulation of exosomes based on size using only 4.2 mW of input laser power. The proposed platform opens up a promising approach for stabilizing single exosomes in solution and controlling their distribution based on size without the risk of photo-induced damage.

Graphical abstract: Exosomes trapping, manipulation and size-based separation using opto-thermo-electrohydrodynamic tweezers

Supplementary files

Article information

Article type
Paper
Submitted
13 fev 2023
Accepted
03 mai 2023
First published
04 mai 2023
This article is Open Access
Creative Commons BY-NC license

Nanoscale Adv., 2023,5, 2973-2978

Exosomes trapping, manipulation and size-based separation using opto-thermo-electrohydrodynamic tweezers

C. Hong, S. Yang and J. C. Ndukaife, Nanoscale Adv., 2023, 5, 2973 DOI: 10.1039/D3NA00101F

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