Issue 3, 2022

Preparation of mesh-like collagen scaffolds for tissue engineering

Abstract

Collagen is an attractive biomaterial to construct scaffolds for tissue engineering and biomedical applications. Mesh-like collagen scaffolds were prepared using a synthetic poly(D,L-lactic-co-glycolic acid) (PLGA) mesh as a template. Collagen microsponges were introduced in the openings of a PLGA mesh to prepare a PLGA–collagen composite mesh, or a collagen coating layer was formed on a PLGA mesh to construct a collagen-coated PLGA mesh. Collagen mesh with large openings (collagen mesh-L) or small openings (collagen mesh-S) was prepared after selective removal of PLGA mesh from the PLGA–collagen composite mesh or collagen-coated PLGA mesh, respectively. The structure of collagen meshes was controlled by the porous structure of precursor meshes. Collagen mesh-S had a hierarchical structure composed of collagen microsponges in the openings of collagen mesh-L. The collagen meshes supported human skin fibroblast adhesion and promoted cell proliferation. In particular, collagen mesh-S showed the highest promotive effects on cell adhesion, homogenous distribution, cell proliferation and dermal tissue regeneration because of its small openings. Fibroblasts cultured in collagen mesh-S expressed various kinds of growth factors that could stimulate the proliferation of fibroblasts and the rapid formation of new dermal tissue. Homogenous dermal tissue was regenerated when fibroblasts were cultured in collagen mesh-S. The template method provides a useful method for mesh-like collagen scaffold preparation.

Graphical abstract: Preparation of mesh-like collagen scaffolds for tissue engineering

Article information

Article type
Paper
Submitted
09 dez 2021
Accepted
17 dez 2021
First published
18 dez 2021
This article is Open Access
Creative Commons BY-NC license

Mater. Adv., 2022,3, 1556-1564

Preparation of mesh-like collagen scaffolds for tissue engineering

Y. Xie, N. Kawazoe, Y. Yang and G. Chen, Mater. Adv., 2022, 3, 1556 DOI: 10.1039/D1MA01166A

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements